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Secondary metabolites from Bacillus amyloliquefaciens isolated from soil can kill Burkholderia pseudomallei

机译：从土壤中分离出的解淀粉芽孢杆菌的次生代谢产物可以杀死假苹果伯克霍尔德氏菌

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摘要

Bacillus species are Gram-positive bacteria found in abundance in nature and their secondary metabolites were found to possess various potential activities, notably antimicrobial. In this study, Bacillus amyloliquefaciens N2-4 and N3-8 were isolated from soil and their metabolites could kill Burkholderia pseudomallei, a Gram-negative pathogenic bacterium also found in soil in its endemic areas. Moreover, the metabolites were able to kill drug resistant isolates of B. pseudomallei and also inhibit other pathogenic bacteria such as Staphylococcus aureus, Escherichia coli and Acinetobacter baumannii but not the non-pathogenic Burkholderia thailandensis, which is closely related to B. pseudomallei. Since the antimicrobial activity of N3-8 was not partially decreased or abolished when treated with proteolytic enzymes or autoclaved, but N2-4 was, these two strains should have produced different compounds. The N3-8 metabolites with antimicrobial activity consisted of both protein and non-protein compounds. The inhibition spectrum of the precipitated proteins compared to the culture supernatant indicated a possible synergistic effect of the non-protein and peptide compounds of N3-8 isolates against other pathogens. When either N2-4 or N3-8 isolates was co-cultured with B. pseudomallei the numbers of the bacteria decreased by 5 log10 within 72 h. Further purification and characterization of the metabolites is required for future use of the bacteria or their metabolites as biological controls of B. pseudomallei in the environment or for development as new drugs for problematic pathogenic bacteria.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-016-0302-0) contains supplementary material, which is available to authorized users.
机译：芽孢杆菌属物种是自然界中发现的大量革兰氏阳性细菌，其次生代谢产物具有多种潜在的活性，尤其是抗菌活性。在这项研究中，从土壤中分离出解淀粉芽孢杆菌N2-4和N3-8，它们的代谢产物可以杀死Burkholderia pseudomallei，这也是在其流行地区的土壤中也发现的革兰氏阴性致病菌。此外，这些代谢物能够杀死假单胞菌的耐药菌株，并抑制其他致病菌，如金黄色葡萄球菌，大肠杆菌和鲍曼不动杆菌，但不能抑制与假单胞菌密切相关的非致病性泰国伯克霍尔德菌。由于用蛋白水解酶或高压灭菌处理时，N3-8的抗菌活性并未部分降低或消失，而N2-4则没有，因此这两个菌株应产生不同的化合物。具有抗菌活性的N3-8代谢物由蛋白质和非蛋白质化合物组成。与培养上清液相比，沉淀蛋白的抑制谱表明N3-8分离株的非蛋白和肽化合物对其他病原体可能具有协同作用。当N2-4或N3-8分离株与假芽孢杆菌共培养时，细菌数量在72小时内减少了5 log10。为了将来将细菌或其代谢物用作假双歧芽孢杆菌在环境中的生物学控制或作为有问题的病原菌的新药开发，需要进一步纯化和表征代谢产物。电子补充材料本文的在线版本（doi： 10.1186 / s13568-016-0302-0）包含补充材料，授权用户可以使用。

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