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Aroma volatile analyses and 2AP characterization at various developmental stages in Basmati and Non-Basmati scented rice (Oryza sativa L.) cultivars

机译:印度香米和非印度香米品种在不同发育阶段的香气挥发性分析和2AP表征

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摘要

BackgroundRice plant growth is comprised of distinct phases, such as vegetative, reproductive, grain filling and maturity phases. In these phases synthesis and availability of primary and secondary metabolites including volatile organic compounds (VOC’s) is highly variable. In scented rice, aroma volatiles are synthesized in aerial plant parts and deposited in mature grains. There are more than 100 VOCs reported to be responsible for flavor in basmati rice. It will be interesting to keep track of aroma volatiles across the developmental stages in scented rice. Therefore, the aroma volatiles contributing in aroma with special reference to the major compound 2 acetyl-1-pyrroline (2AP) were screened at seven developmental stages in scented rice cultivars Basmati-370 and Ambemohar-157 along with non-scented rice cultivar IR-64 as a control following HS-SPME-GC-MS method. In addition, the expression levels of key genes and precursor levels involved in 2AP biosynthesis were studied.
机译:背景技术水稻植物的生长由不同的阶段组成,例如营养,生殖,籽粒充实和成熟阶段。在这些阶段中,主要和次要代谢物(包括挥发性有机化合物(VOC))的合成和可用性变化很大。在香米中,香气挥发物在空中植物部位合成并沉积在成熟谷物中。据报道,巴斯马蒂大米中有100多种VOC负责风味。跟踪香米在整个发育阶段的香气挥发将是很有趣的。因此,在有香味的水稻品种Basmati-370和Ambemohar-157以及无香味的水稻品种IR-中,在七个发育阶段筛选了主要涉及主要化合物2乙酰基-1-吡咯啉(2AP)的香气挥发物。遵循HS-SPME-GC-MS方法的64位控件。此外,还研究了2AP生物合成中关键基因的表达水平和前体水平。

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