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首页> 美国卫生研究院文献>Journal of Insect Science >Cloning and Expression Analysis of Four Heat Shock Protein Genes inEricerus pela(Homoptera: Coccidae)
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Cloning and Expression Analysis of Four Heat Shock Protein Genes inEricerus pela(Homoptera: Coccidae)

机译：大豆4个热休克蛋白基因的克隆与表达分析。埃里克斯·佩拉（同翅目：球虫）

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To explore the function of small heat shock protein genes ( shsp s) and hsp70 in Ericerus pela , we cloned the full-length cDNA sequences of hsp21.5 , hsp21.7 , hsp70 , and hsc70 and the genomic sequence of hsc70. Open reading frames of the fourhsps were 570, 564, 1,908, and 1,962 base pairs (bp), respectively, which encode proteins with calculated molecular mass of 21.5, 21.7, 69.8, and 71.6 kDa. Amino acid sequence analysis revealed the presence of the conserved Hsp motifs in all four proteins. The genomic DNA ofhsc70had four introns.ep-hsp21.5was orthologous andep-hsp21.7was species specific. Expression of all four transcripts during heat or cold stress and development was examined by quantitative real-time polymerase chain reaction. All fourhsps were upregulated during heat or cold stress in female adults, indicating a correlation between the fourhsps and heat or cold-stress tolerance in female adults.ep-hsp21.7andep-hsp70were upregulated during heat stress in male larvae, implying a correlation between the twohsps and heat-stress tolerance in male larvae. The fourep-hsps were also upregulated during the developmental process in males, andep-hsp21.5,ep-hsp70, andep-hsc70were upregulated in females, which indicates their possible role in the developmental regulation ofE. pela.
机译：为了探索小热休克蛋白基因（shsp s）和hsp70在埃里克勒斯贝拉中的功能，我们克隆了hsp21.5，hsp21.7，hsp70和hsc70的全长cDNA序列以及hsc70的基因组序列。打开四个阅读框热休克蛋白分别为570、564、1,908和1,962个碱基对（bp），它们编码的蛋白质的计算分子量为21.5、21.7、69.8和71.6 kDa。氨基酸序列分析揭示了在所有四种蛋白质中均存在保守的Hsp基序。的基因组DNAhsc70有四个内含子。ep-hsp21.5是直系同源的ep-hsp21.7是特定于物种的。通过定量实时聚合酶链反应检查在热或冷胁迫和发育过程中所有四个转录物的表达。全部四个热休克蛋白在成年女性的热或冷压力下，它们的上调，表明这四个之间的相关性热休克蛋白对成年女性的耐高温和耐冷压力。ep-hsp21.7和ep-hsp70在雄性幼虫热应激过程中被上调，这表明两者之间存在相关性 hsp 和对幼虫的热应激耐受性。四个 ep-hsp 男性在发育过程中也被上调，并且 ep-hsp21.5 ， ep-hsp70 和 ep-hsc70 在女性中被上调，这表明它们可能在女性的发育调节中发挥作用 E。佩拉。

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