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首页> 美国卫生研究院文献>Journal of Insect Science >Gene Expression Profiling in the Thiamethoxam Resistant and Susceptible B-biotype Sweetpotato Whitefly Bemisia tabaci
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Gene Expression Profiling in the Thiamethoxam Resistant and Susceptible B-biotype Sweetpotato Whitefly Bemisia tabaci

机译：耐噻虫嗪和易感B-生物型甘薯粉虱烟粉虱的基因表达谱分析

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摘要

Thiamethoxam has been used as a major insecticide to control the B-biotype sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). Due to its excessive use, a high level of resistance to thiamethoxam has developed worldwide over the past several years. To better understand the molecular mechanisms underlying this resistance in B. tabaci, gene profiles between the thiamethoxam-resistant and thiamethoxam-susceptible strains were investigated using the suppression subtractive hybridization (SSH) library approach. A total of 72 and 52 upand down-regulated genes were obtained from the forward and reverse SSH libraries, respectively. These expressed sequence tags (ESTs) belong to several functional categories based on their gene ontology annotation. Some categories such as cell communication, response to abiotic stimulus, lipid particle, and nuclear envelope were identified only in the forward library of thiamethoxam-resistant strains. In contrast, categories such as behavior, cell proliferation, nutrient reservoir activity, sequence-specific DNA binding transcription factor activity, and signal transducer activity were identified solely in the reverse library.To study the validity of the SSH method, 16 differentially expressed genes from both forward and reverse SSH libraries were selected randomly for further analyses using quantitative realtime PCR (qRT-PCR). The qRT-PCR results were fairly consistent with the SSH results; however, only 50% of the genes showed significantly different expression profiles between the thiamethoxam-resistant and thiamethoxam-susceptible whiteflies. Among these genes, a putative NAD-dependent methanol dehydrogenase was substantially over-expressed in the thiamethoxamresistant adults compared to their susceptible counterparts. The distributed profiles show that it was highly expressed during the egg stage, and was most abundant in the abdomen of adult females.
机译：噻虫嗪已被用作控制B生物型甘薯粉虱烟粉虱（Bemisia tabaci（Gennadius）（Hemiptera：Aleyrodidae）的主要杀虫剂。由于过量使用，在过去的几年中，全球范围内对噻虫嗪产生了高度的抗药性。为了更好地了解烟粉虱这种抗药性的分子机制，使用抑制消减杂交（SSH）文库方法研究了噻虫嗪抗药性与噻虫嗪易感菌株之间的基因谱。从正向和反向SSH库分别获得了72和52个上调和下调的基因。这些表达的序列标签（EST）基于它们的基因本体注释属于几个功能类别。某些类别，例如细胞通讯，对非生物刺激的反应，脂质颗粒和核被膜，仅在抗噻虫嗪菌株的正向文库中鉴定。相比之下，仅在反向文库中鉴定了行为，细胞增殖，营养储库活性，序列特异性DNA结合转录因子活性和信号转导活性等类别。为了研究SSH方法的有效性，从SSH中分离了16个差异表达基因使用定量实时PCR（qRT-PCR）随机选择正向和反向SSH库进行进一步分析。 qRT-PCR结果与SSH结果相当一致。然而，只有50％的基因在耐噻虫嗪和易感噻虫的粉虱之间显示出明显不同的表达特征。在这些基因中，与易感人群相比，在对噻虫嗪抗性的成年人中，NAD依赖性的依赖于甲醇的脱氢酶明显过表达。分布图表明它在卵期高表达，并且在成年雌性的腹部中含量最高。

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