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The geographic mosaic of herbicide resistance evolution in the common morning glory Ipomoea purpurea: Evidence for resistance hotspots and low genetic differentiation across the landscape

机译:常见牵牛花紫菜(Ipomoea purpurea)中除草剂抗性进化的地理镶嵌图:整个景观中抗药性热点和低遗传分化的证据

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摘要

Strong human-mediated selection via herbicide application in agroecosystems has repeatedly led to the evolution of resistance in weedy plants. Although resistance can occur among separate populations of a species across the landscape, the spatial scale of resistance in many weeds is often left unexamined. We assessed the potential that resistance to the herbicide glyphosate in the agricultural weed Ipomoea purpurea has evolved independently multiple times across its North American range. We examined both adaptive and neutral genetic variations in 44 populations of I. purpurea by pairing a replicated dose–response greenhouse experiment with SSR genotyping of experimental individuals. We uncovered a mosaic pattern of resistance across the landscape, with some populations exhibiting high-survival postherbicide and other populations showing high death. SSR genotyping revealed little evidence of isolation by distance and very little neutral genetic structure associated with geography. An approximate Bayesian computation (ABC) analysis uncovered evidence for migration and admixture among populations before the widespread use of glyphosate rather than the very recent contemporary gene flow. The pattern of adaptive and neutral genetic variations indicates that resistance in this mixed-mating weed species appears to have evolved in independent hotspots rather than through transmission of resistance alleles across the landscape.
机译:在农业生态系统中,通过除草剂的强力人类介导选择已反复导致杂草植物产生抗药性。尽管抗性可能发生在整个景观的不同物种种群之间,但许多杂草中的抗性空间尺度常常未被检查。我们评估了在农业杂草紫菜中对除草剂草甘膦的抗性在其北美范围内独立进化多次的可能性。通过将重复的剂量反应温室试验与实验个体的SSR基因型配对,我们研究了紫pu的44个种群的适应性遗传变异和中性遗传变异。我们在整个景观中发现了抗药性的马赛克图案,其中一些种群表现出高存活率的除草剂,而其他种群表现出高死亡率。 SSR基因分型几乎没有证据表明可以通过距离进行分离,也几乎没有与地理相关的中性遗传结构。近似的贝叶斯计算(ABC)分析揭示了在草甘膦广泛使用之前而不是最近的现代基因流动之前种群之间迁移和混合的证据。适应性和中性遗传变异的模式表明,这种杂交杂草物种的抗性似乎是在独立的热点中进化的,而不是通过抗性等位基因在整个景观中的传播。

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