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Dynamics of marine bacterial community diversity of the coastal waters of the reefs inlets and wastewater outfalls of southeast Florida

机译：佛罗里达州东南部珊瑚礁进水口和废水口沿海水域海洋细菌群落多样性的动态

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Coastal waters adjacent to populated southeast Florida possess different habitats (reefs, oceanic inlets, sewage outfalls) that may affect the composition of their inherent microbiomes. To determine variation according to site, season, and depth, over the course of 1 year, we characterized the bacterioplankton communities within 38 nearshore seawater samples derived from the Florida Area Coastal Environment (FACE) water quality survey. Six distinct coastal locales were profiled – the Port Everglades and Hillsboro Inlets, Hollywood and Broward wastewater outfalls, and associated reef sites using culture-independent, high-throughput pyrosequencing of the 16S rRNA V4 region. More than 227,000 sequences helped describe longitudinal taxonomic profiles of marine bacteria and archaea. There were 4447 unique operational taxonomic units (OTUs) identified with a mean OTU count of 5986 OTUs across all sites. Bacterial taxa varied significantly by season and by site using weighted and unweighted Unifrac, but depth was only supported by weighted Unifrac, suggesting a change due to presence/absence of certain OTUs. Abundant microbial taxa across all samples included Synechococcus, Pelagibacteraceae, Bacteroidetes, and various Proteobacteria. Unifrac analysis confirmed significant differences at inlet sites relative to reef and outfalls. Inlet-based bacterioplankton significantly differed in greater abundances of Rhodobacteraceae and Cryomorphaceae, and depletion of SAR406 sequences. This study also found higher counts of Firmicutes, Chloroflexi, and wastewater associated SBR1093 bacteria at the outfall and reef sites compared to inlet sites. This study profiles local bacterioplankton populations in a much broader context, beyond culturing and quantitative PCR, and expands upon the work completed by the National Oceanic and Atmospheric Administration FACE program.
机译：与人口稠密的佛罗里达东南部相邻的沿海水域具有不同的栖息地（礁石，海洋入口，排污口），这可能会影响其固有微生物群落的组成。为了确定地点，季节和深度的变化，在一年的过程中，我们对来自佛罗里达州沿海环境（FACE）水质调查的38个近岸海水样品中的浮游细菌群落进行了特征分析。使用16S rRNA V4区域的与培养无关的高通量焦磷酸测序技术，对六个不同的沿海地区进行了概述-沼泽地港和希尔斯伯勒湾入口，好莱坞和布劳沃德废水排放口以及相关的珊瑚礁地点。超过227,000个序列帮助描述了海洋细菌和古细菌的纵向分类学概况。在所有站点中，共有4447个唯一的操作分类单位（OTU），平均OTU计数为5986个OTU。使用加权和未加权Unifrac，细菌分类群随季节和地点而变化很大，但是深度仅由加权Unifrac支持，表明由于某些OTU的存在/不存在而导致的变化。所有样本中都有丰富的微生物类群，包括Synechococcus，Pelagibacteraceae，Bacteroidetes和各种Proteobacteria。 Unifrac分析证实，相对于礁石和排污口，进口处存在显着差异。基于入口的浮游植物在红景天科和低温科的丰度更高以及SAR406序列耗竭方面差异显着。这项研究还发现，与进水口相比，出水口和礁石位处的沙门氏菌，绿藻和与废水相关的SBR1093细菌数量更高。这项研究在培养和定量PCR之外的更广泛的背景下，对当地浮游细菌种群进行了分析，并扩展了美国国家海洋与大气管理局FACE计划完成的工作。

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