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首页> 美国卫生研究院文献>Microbial Biotechnology >The Rsm regulon of plant growth-promoting Pseudomonas fluorescens SS101: role of small RNAs in regulation of lipopeptide biosynthesis
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The Rsm regulon of plant growth-promoting Pseudomonas fluorescens SS101: role of small RNAs in regulation of lipopeptide biosynthesis

机译：促进植物生长的荧光假单胞菌SS101的Rsm调控：小RNA在脂肽生物合成调控中的作用

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The rhizobacterium Pseudomonas fluorescens SS101 inhibits growth of oomycete and fungal pathogens, and induces resistance in plants against pathogens and insects. To unravel regulatory pathways of secondary metabolite production in SS101, we conducted a genome-wide search for sRNAs and performed transcriptomic analyses to identify genes associated with the Rsm (repressor of secondary metabolites) regulon. In silico analysis led to the identification of 16 putative sRNAs in the SS101 genome. In frame deletion of the sRNAs rsmY and rsmZ showed that the Rsm system regulates the biosynthesis of the lipopeptide massetolide A and involves the two repressor proteins RsmA and RsmE, with the LuxR-type transcriptional regulator MassAR as their most likely target. Transcriptome analyses of the rsmYZ mutant further revealed that genes associated with iron acquisition, motility and chemotaxis were significantly upregulated, whereas genes of the type VI secretion system were downregulated. Comparative transcriptomic analyses showed that most, but not all, of the genes controlled by RsmY/RsmZ are also controlled by the GacS/GacA two-component system. We conclude that the Rsm regulon of P. fluorescens SS101 plays a critical role in the regulation of lipopeptide biosynthesis and controls the expression of other genes involved in motility, competition and survival in the plant rhizosphere.
机译：荧光假单胞菌（Pseudomonas fluorescens）SS101抑制卵菌和真菌病原体的生长，并诱导植物对病原体和昆虫的抗性。为了揭示SS101中次级代谢产物产生的调控途径，我们进行了全基因组搜索sRNA，并进行了转录组学分析，以鉴定与Rsm（次级代谢产物阻遏物）调节子相关的基因。电子计算机分析导致在SS101基因组中鉴定出16个假定的sRNA。在sRNA rsmY和rsmZ的框内缺失显示Rsm系统调节脂肽Massetolide A的生物合成，并涉及两个阻遏蛋白RsmA和RsmE，其中LuxR型转录调节因子MassAR为最可能的靶标。 rsmYZ突变体的转录组分析进一步表明，与铁的获取，运动和趋化性相关的基因被显着上调，而VI型分泌系统的基因被下调。对比转录组分析显示，受RsmY / RsmZ控制的大多数（但不是全部）基因也受GacS / GacA两组分系统控制。我们得出结论，P。fluorescens SS101的Rsm调控基因在脂肽生物合成的调控中起着关键作用，并控制植物根际中参与运动性，竞争和存活的其他基因的表达。

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