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首页> 美国卫生研究院文献>Ecology and Evolution >Nucleotide diversity of vernalization and flowering-time-related genes in a germplasm collection of meadow fescue (Festuca pratensis Huds. syn. Lolium pratense (Huds.) Darbysh.)
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Nucleotide diversity of vernalization and flowering-time-related genes in a germplasm collection of meadow fescue (Festuca pratensis Huds. syn. Lolium pratense (Huds.) Darbysh.)

机译：草甸羊茅种质资源中春化和开花时间相关基因的核苷酸多样性（Festuca pratensis Huds。syn。Lolium pratense（Huds。）Darbysh。）

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In plant species, control of flowering time is an important factor for adaptation to local natural environments. The Vrn1, CO, FT1 and CK2α genes are key components in the flowering-specific signaling pathway of grass species. Meadow fescue is an agronomically important forage grass species, which is naturally distributed across Europe and Western Asia. In this study, meadow fescue flowering-time-related genes were resequenced to assess nucleotide diversity in European and Western Asian subpopulations. Identified sequence polymorphisms were then converted into PCR-based molecular genetic markers, and a meadow fescue germplasm collection was genotyped to investigate global allelic variation. Lower nucleotide diversities were observed for the Vrn1 and CO orthologs, while relatively higher values were observed for the FT1 and casein kinase II α-subunit (CK2α) orthologs. The nucleotide diversity for FT1 orthologs in the Western Asian subpopulation was significantly higher than those of the European subpopulation. Similarly, significant differences in nucleotide diversity for the remaining genes were observed between several combinations of subpopulation. The global allele distribution pattern was consistent with observed level of nucleotide diversity. These results suggested that the degree of purifying selection acting on the genes differs according to geographical location. As previously shown for model plant species, functional specificities of flowering-time-related genes may also vary according to environmental conditions.
机译：在植物物种中，控制开花时间是适应当地自然环境的重要因素。 Vrn1，CO，FT1和CK2α基因是草种开花特异性信号通路中的关键成分。草甸羊茅是一种在农业上重要的牧草，在欧洲和西亚自然分布。在这项研究中，草地羊茅花时间相关的基因被重新测序，以评估欧洲和西亚亚人群的核苷酸多样性。然后将已鉴定的序列多态性转化为基于PCR的分子遗传标记，并对草甸羊茅种质收集物进行基因分型以研究总体等位基因变异。 Vrn1和CO直向同源物观察到较低的核苷酸多样性，而FT1和酪蛋白激酶IIα亚基（CK2α）直向同源物观察到相对较高的值。西亚亚群中FT1直系同源物的核苷酸多样性显着高于欧洲亚群。类似地，在亚群的几种组合之间观察到其余基因的核苷酸多样性的显着差异。全球等位基因分布模式与观察到的核苷酸多样性水平相符。这些结果表明，作用于基因的纯化选择的程度根据地理位置而不同。如先前针对模型植物物种所示，开花时间相关基因的功能特异性也可能根据环境条件而变化。

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