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Biosurfactant-mediated biodegradation of straight and methyl-branched alkanes by Pseudomonas aeruginosa ATCC 55925

机译:铜绿假单胞菌ATCC 55925对表面活性剂介导的直链和甲基支链烷烃的生物降解

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摘要

Accidental oil spills and waste disposal are important sources for environmental pollution. We investigated the biodegradation of alkanes by Pseudomonas aeruginosa ATCC 55925 in relation to a rhamnolipid surfactant produced by the same bacterial strain. Results showed that the linear C11-C21 compounds in a heating oil sample degraded from 6% to 100%, whereas the iso-alkanes tended to be recalcitrant unless they were exposed to the biosurfactant; under such condition total biodegradation was achieved. Only the biodegradation of the commercial C12-C19 alkanes could be demonstrated, ranging from 23% to 100%, depending on the experimental conditions. Pristane (a C19 branched alkane) only biodegraded when present alone with the biosurfactant and when included in an artificial mixture even without the biosurfactant. In all cases the biosurfactant significantly enhanced biodegradation. The electron scanning microscopy showed that cells depicted several adaptations to growth on hydrocarbons, such as biopolymeric spheres with embedded cells distributed over different layers on the spherical surfaces and cells linked to each other by extracellular appendages. Electron transmission microscopy revealed transparent inclusions, which were associated with hydrocarbon based-culture cells. These patterns of hydrocarbon biodegradation and cell adaptations depended on the substrate bioavailability, type and length of hydrocarbon.
机译:意外漏油和废物处置是造成环境污染的重要来源。我们调查了铜绿假单胞菌ATCC 55925对由同一细菌菌株产生的鼠李糖脂表面活性剂对烷烃的生物降解作用。结果表明,取暖油样品中的线性C11-C21化合物从6%降解为100%,而异链烷烃除非具有生物表面活性剂,否则趋向于难分解。在这种条件下,实现了总生物降解。根据实验条件,仅能证明商业C12-C19烷烃的生物降解范围为23%至100%。 rist烷(一种C19支链烷烃)仅在与生物表面活性剂一起存在时,甚至在没有生物表面活性剂的情况下包含在人工混合物中时也可被生物降解。在所有情况下,生物表面活性剂均显着增强了生物降解能力。电子扫描显微镜显示,细胞表现出对碳氢化合物生长的几种适应,例如生物聚合物球体,其嵌入的细胞分布在球形表面的不同层上,并且细胞通过细胞外附属物相互连接。电子透射显微镜显示透明夹杂物,其与基于烃的培养细胞有关。碳氢化合物生物降解和细胞适应的这些模式取决于底物的生物利用度,碳氢化合物的类型和长度。

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