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首页> 美国卫生研究院文献>Evidence-based Complementary and Alternative Medicine : eCAM >Neuroprotective Activity of Sibjeondaebo-tang on Aβ Peptide-Induced Damages
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Neuroprotective Activity of Sibjeondaebo-tang on Aβ Peptide-Induced Damages

机译：西伯定onda汤对Aβ肽诱导的损伤的神经保护活性。

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Background. Sibjeondaebo-tang (SJDBT) has been used to treat diverse disorders including neuropsychiatric disabilities in traditional Korean medicine. Objective. The present study aims to investigate the potential effects of SJDBT on neuroprotection against Aβ peptide-induced damage using in vitro culture and in vivo rat brain systems. Materials and Methods. PC12 cell viability was analyzed by MTT assay, and neurite arborizations and caspase 3 protein signals in cultured PC12 cells and in vivo cortical neurons were analyzed by immunofluorescence staining. Phospho-Erk1/2 protein was analyzed by immunofluorescence staining and western blot analysis. Results. In PC12 cells, atrophied cell body and reduced neurite extension by Aβ treatment were recovered by SJDBT treatment. Caspase 3 protein signals were increased in Aβ-treated PC12 cells, but SJDBT treatment decreased apoptotic cell death. Caspase 3 activation in cortical neurons, which was induced similarly by Aβ treatment, was reduced by SJDBT treatment. Furthermore, phospho-Erk1/2 protein levels, which had been decreased by Aβ treatment, were elevated in the cortical neurons by SJDBT treatment. Conclusion. These data show that SJDBT may play a role in protecting from damages induced by Aβ in neuronal tissue and further suggest that SJDBT can be explored as the potential therapeutic target for AD treatments in human.
机译：背景。 Sibjeondaebo-tang（SJDBT）已被用来治疗包括韩国传统医学在内的多种疾病，包括神经精神疾病。目的。本研究旨在研究SJDBT对使用体外培养和体内大鼠脑系统对抗Aβ肽诱导的损伤的神经保护的潜在作用。材料和方法。通过MTT分析法分析PC12细胞的生存力，并通过免疫荧光染色分析培养的PC12细胞和体内皮层神经元中的神经突节和半胱天冬酶3蛋白信号。通过免疫荧光染色和蛋白质印迹分析来分析磷酸化-Erk1 / 2蛋白。结果。在PC12细胞中，通过SJDBT处理可恢复萎缩的细胞体和通过Aβ处理减少的神经突延伸。 Caspase 3蛋白信号在Aβ处理的PC12细胞中增加，但SJDBT处理降低了凋亡细胞的死亡。 SJDBT处理可减少Aβ处理诱导的皮质神经元中的Caspase 3活化。此外，通过SJDBT处理，通过Aβ处理降低的磷酸化Erk1 / 2蛋白水平在皮质神经元中升高。结论。这些数据表明，SJDBT可能在保护免受神经元组织中Aβ诱导的损伤中起作用，并且进一步表明，SJDBT可以被探索为人AD治疗的潜在治疗靶标。

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