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TeoNAM: A Nested Association Mapping Population for Domestication and Agronomic Trait Analysis in Maize

机译:TeoNAM:用于玉米驯化和农艺性状分析的嵌套关联图种群

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摘要

Recombinant inbred lines (RILs) are an important resource for mapping genes controlling complex traits in many species. While RIL populations have been developed for maize, a maize RIL population with multiple teosinte inbred lines as parents has been lacking. Here, we report a teosinte nested association mapping (TeoNAM) population, derived from crossing five teosinte inbreds to the maize inbred line W22. The resulting 1257 BC1S4 RILs were genotyped with 51,544 SNPs, providing a high-density genetic map with a length of 1540 cM. On average, each RIL is 15% homozygous teosinte and 8% heterozygous. We performed joint linkage mapping (JLM) and a genome-wide association study (GWAS) for 22 domestication and agronomic traits. A total of 255 QTL from JLM were identified, with many of these mapping near known genes or novel candidate genes. TeoNAM is a useful resource for QTL mapping for the discovery of novel allelic variation from teosinte. TeoNAM provides the first report that PROSTRATE GROWTH1, a rice domestication gene, is also a QTL associated with tillering in teosinte and maize. We detected multiple QTL for flowering time and other traits for which the teosinte allele contributes to a more maize-like phenotype. Such QTL could be valuable in maize improvement.
机译:重组自交系(RIL)是绘制许多物种中控制复杂性状的基因的重要资源。尽管已经为玉米开发了RIL种群,但一直缺乏作为亲本的具有多个teosinte自交系的玉米RIL种群。在这里,我们报告了一个teininte巢式关联映射(TeoNAM)种群,该种群是从五个teosinte自交系与玉米自交系W22杂交而来的。对得到的1257个BC1S4 RIL进行了51,544个SNP的基因分型,从而提供了一个高密度的遗传图谱,其长度为1540 cM。平均而言,每个RIL是15%纯合的肌腱蛋白和8%杂合的。我们进行了22种驯化和农艺性状的联合连锁作图(JLM)和全基因组关联研究(GWAS)。从JLM总共鉴定出255个QTL,其中许多定位在已知基因或新候选基因附近。 TeoNAM是用于QTL映射的有用资源,用于发现teosinte的新等位基因变异。 TeoNAM提供了第一个报告,即水稻驯化基因PROSTRATE GROWTH1也是与Teosinte和玉米分till相关的QTL。我们检测到多个QTL的开花时间和其他特征,其中teosinte等位基因导致了更多的玉米样表型。这样的QTL在玉米改良中可能是有价值的。

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