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Proteome turnover in the bloodstream and procyclic forms ofTrypanosoma brucei measured by quantitative proteomics

机译：蛋白质组在血液中的周转和脯氨酸的形式定量蛋白质组学测定布鲁氏锥虫

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>Background: Cellular proteins vary significantly in both abundance and turnover rates. These parameters depend upon their rates of synthesis and degradation and it is useful to have access to data on protein turnover rates when, for example, designing genetic knock-down experiments or assessing the potential usefulness of covalent enzyme inhibitors. Little is known about the nature and regulation of protein turnover in Trypanosoma brucei, the etiological agent of human and animal African trypanosomiasis. >Methods: To establish baseline data on T. brucei proteome turnover, a Stable Isotope Labelling with Amino acids in Cell culture (SILAC)-based mass spectrometry analysis was performed to reveal the synthesis and degradation profiles for thousands of proteins in the bloodstream and procyclic forms of this parasite. >Results: This analysis revealed a slower average turnover rate of the procyclic form proteome relative to the bloodstream proteome. As expected, many of the proteins with the fastest turnover rates have functions in the cell cycle and in the regulation of cytokinesis in both bloodstream and procyclic forms. Moreover, the cellular localization of T. brucei proteins correlates with their turnover, with mitochondrial and glycosomal proteins exhibiting slower than average turnover rates. >Conclusions: The intention of this study is to provide the trypanosome research community with a resource for protein turnover data for any protein or group of proteins. To this end, bioinformatic analyses of these data are made available via an open-access web resource with data visualization functions.
机译：>背景：细胞蛋白质的丰度和周转率差异很大。这些参数取决于它们的合成和降解速率，例如在设计基因敲除实验或评估共价酶抑制剂的潜在用途时，获取有关蛋白质周转率的数据非常有用。关于布鲁氏锥虫（人类和动物非洲锥虫病的病原体）中蛋白质更新的性质和调控知之甚少。 >方法：为了建立布鲁氏杆菌蛋白质组更新的基线数据，在基于细胞培养（SILAC）的质谱分析中进行了氨基酸稳定同位素标记，以揭示数千种蛋白质的合成和降解情况。血液中的蛋白质和这种寄生虫的前环形式。 >结果：该分析表明，前环形式的蛋白质组的平均周转率相对于血流蛋白质组而言要慢。如所期望的，许多具有最快周转率的蛋白质在细胞周期中以及在血流和前环形式的胞质分裂的调节中具有功能。此外，布鲁氏杆菌蛋白的细胞定位与其周转率相关，线粒体和糖体蛋白的周转率低于平均水平。 >结论：这项研究的目的是为锥虫研究界提供任何蛋白质或蛋白质组蛋白质更新数据的资源。为此，可通过具有数据可视化功能的开放式网络资源来对这些数据进行生物信息学分析。

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