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首页> 美国卫生研究院文献>other >Fine Mapping of Lycopene Content and Flesh Color Related Gene and Development of Molecular Marker–Assisted Selection for Flesh Color in Watermelon (Citrullus lanatus)
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Fine Mapping of Lycopene Content and Flesh Color Related Gene and Development of Molecular Marker–Assisted Selection for Flesh Color in Watermelon (Citrullus lanatus)

机译：番茄红素含量和果肉相关基因的精细定位及西瓜果肉颜色分子标记辅助选择的开发

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摘要

Lycopene content and flesh color are important traits determined by a network of carotenoid metabolic pathways in watermelon. Based on our previous study of genetic inheritance and initial mapping using F2 populations of LSW-177 (red flesh) × cream of Saskatchewan (pale yellow flesh), red flesh color was controlled by one recessive gene regulating red and pale yellow pigmentation, and a candidate region related to lycopene content was detected spanning a 392,077-bp region on chromosome 4. To obtain a more precise result for further study, three genetic populations and a natural panel of 81 watermelon accessions with different flesh colors were used in this research. Herein, we narrowed the preliminary mapping region to 41,233 bp with the linkage map generated from F2 populations of LSW-177 (red flesh) × cream of Saskatchewan (pale yellow flesh) with 1,202 individuals. Two candidate genes, Cla005011 and Cla005012, were found in the fine mapping region; therein Cla005011 was a key locus annotated as a lycopene β-cyclase gene. Phylogenetic tree analysis showed that Cla005011 was the closest relative gene in gourd. LSW-177 × PI 186490 (white flesh) and another BC1 population derived from garden female (red flesh) × PI 186490 were generated to verify the accuracy of the red flesh candidate gene region. By analyzing the expression levels of candidate genes in different developmental stages of different color watermelon varieties, Cla005011 for the expression differences was not the main reason for the flesh color variation between COS and LSW-177. This indicated that the LCYB gene might regulate fruit color changes at the protein level. A new marker-assisted selection system to identify red and yellow flesh colors in watermelon was developed with flesh color–specific CAPS markers and tested in 81 watermelon accessions.
机译：番茄红素含量和果肉颜色是由西瓜中类胡萝卜素代谢途径网络确定的重要特征。根据我们先前对LSW-177（红色果肉）×Saskatchewan（淡黄色果肉）霜的F2种群的遗传遗传和初始作图研究，红色果肉的颜色由一个隐性基因控制，该隐性基因调节红色和淡黄色色素沉着，在4号染色体上的392,077-bp区域中检测到了与番茄红素含量有关的候选区域。为了获得更精确的结果以供进一步研究，本研究使用了3个遗传种群和81种不同果肉颜色的西瓜天然成分。在这里，我们将LSW-177（红肉）×Saskatchewan奶油（浅黄肉）的F2种群的F2种群产生的连锁图谱将初步定位区域缩小到41,233 bp，共有1,202个人。在精细作图区域发现了两个候选基因Cla005011和Cla005012。其中Cla005011是注释为番茄红素β-环化酶基因的关键基因座。系统进化树分析表明，Cla005011是葫芦中最接近的相对基因。生成了LSW-177×PI 186490（白色果肉）和另一个源自花园雌性（红色果肉）×PI 186490的BC1种群，以验证红色果肉候选基因区域的准确性。通过分析不同颜色西瓜品种不同发育阶段候选基因的表达水平，Cla005011表达差异并不是COS和LSW-177果肉颜色变化的主要原因。这表明LCYB基因可能在蛋白质水平上调节果实的颜色变化。开发了一种新的标记辅助选择系统，用于识别西瓜中的红色和黄色果肉，并使用特定于肤色的CAPS标记，并在81个西瓜种中进行了测试。

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