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首页> 美国卫生研究院文献>other >BIOF–HILO Assay: A New MALDI–TOF Mass Spectrometry Based Method for Discriminating Between High- and Low-Biofilm-Producing Candida parapsilosis Isolates
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BIOF–HILO Assay: A New MALDI–TOF Mass Spectrometry Based Method for Discriminating Between High- and Low-Biofilm-Producing Candida parapsilosis Isolates

机译：BIOF–HILO测定：一种基于MALDI–TOF质谱的新方法用于区分生产高生物膜和低生物膜的假丝酵母念珠菌

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摘要

Candida parapsilosis is the most frequent cause of catheter-related candidemia among non-Candida albicans species. This may be related to intrinsic capabilities as adhering and forming a biofilm on abiotic surfaces such as on medical devices. As previously demonstrated, patients infected with high biofilm-producing C. parapsilosis isolates had a greater mortality risk compared to patients infected with low biofilm-producing C. parapsilosis isolates. We developed the BIOF–HILO assay, a MALDI–TOF mass spectrometry (MS)-based assay, which compares mass spectra obtained from attached and suspended isolate cells during the early (i.e., 3-h) adhesion phase of in vitro biofilm formation. The composite correlation index (CCI) analysis was used to discriminate between mass spectra differences of the two cell types, classifying all 50 C. parapsilosis clinical isolates, included in the study, after only 3-h of testing, in high or low biofilm producers. All high (n = 25) or low (n = 25) biofilm producers had, according to CCI mass spectra comparison values, higher or lower than one CCI ratios, which were obtained by dividing the CCIsuspended cells by the CCIattached cells. In conclusion, the BIOF–HILO assay allows a rapid categorization of C. parapsilosis clinical isolates in high or low biofilm producers. This information, if timely provided to physicians, may improve treatment outcomes in patients with C. parapsilosis candidemia.
机译：在白色念珠菌中，副念珠菌是引起导管相关念珠菌血症的最常见原因。这可能与在非生物表面（例如医疗设备）上粘附并形成生物膜的固有能力有关。如前所述，感染高生物膜的副产念珠菌分离株感染的患者与感染低生物膜的副产念珠菌分离株感染的患者相比，死亡风险更高。我们开发了基于MALDI-TOF质谱（MS）的BIOF-HILO测定法，该测定法比较了体外生物膜形成的早期（即3小时）粘附阶段从附着和悬浮的分离细胞获得的质谱。仅在进行了3小时的测试后，就使用了高，低生物膜生产商的复合相关指数（CCI）分析来区分两种细胞类型的质谱差异，并对研究中包括的所有50株副寄生虫临床隔离株进行了分类。 。根据CCI质谱图比较值，所有高（n = 25）或低（n = 25）生物膜生产商的生产成本均高于或低于一个CCI比，这是通过将CCI悬浮细胞除以CCI附着细胞而获得的。总之，BIOF–HILO分析可以快速分类高生物膜产量或低生物膜产量的寄生虫角斑病临床分离株。如果及时将这些信息提供给医生，可能会改善C. parapsilosis念珠菌血症患者的治疗效果。

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