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Validation of a simple sample preparation method for multielement analysis of bovine serum

机译：一种用于牛血清多元素分析的简单样品制备方法的验证

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Here we propose a single acid digestion (SAD) sample preparation method for ICP-MS analysis of animal serum samples to determine trace element contents. The method was evaluated in comparison with a commonly used procedure involving dilution of samples in an alkaline solution (AKD). In the SAD procedure, aliquots (1 mL) of bovine serum samples were treated at low temperature with a mixture of concentrated nitric acid and hydrogen peroxide. Trace elements (As, B, Ba, Cd, Co, Cr, Cu, Fe, Hg, Li, Mn, Mo, Ni, Pb, Sb, Se, Sr, U, and Zn) were directly determined by ICP-MS analysis of diluted solutions of samples. Both methods were sufficiently sensitive to enable quantification of most trace elements, with the exception of the AKD method for Cd, Hg and Pb. The quality of the data was verified by using certified reference material. Good results were obtained for the SAD procedure and all elements, but recoveries were unacceptable with the AKD procedure for Se (recovery: 57%), Cd (154%) and Fe (139%). Strong associations (R2>0.90, P = 0.000) between the data obtained by both methods were demonstrated for the elements considered. The proposed SAD sample preparation method produced satisfactory results for determining most toxic and essential trace elements targeted in monitoring studies.
机译：在这里，我们提出了一种用于动物血清样品的ICP-MS分析的单酸消解（SAD）样品制备方法，以确定痕量元素的含量。与常用程序（包括在碱性溶液（AKD）中稀释样品）的比较方法进行了评估。在SAD程序中，将牛血清样品的等分试样（1 mL）在低温下用浓硝酸和过氧化氢的混合物处理。通过ICP-MS分析直接测定痕量元素（As，B，Ba，Cd，Co，Cr，Cu，Fe，Hg，Li，Mn，Mo，Ni，Pb，Sb，Se，Sr，U和Zn）样品的稀释溶液。除了用于镉，汞和铅的AKD方法外，这两种方法都足够灵敏，可以定量大多数痕量元素。数据的质量通过使用认证的参考材料进行了验证。 SAD方法和所有元素均获得了良好的结果，但AKD方法对Se（回收率：57％），Cd（154％）和Fe（139％）的回收率是不可接受的。对于所考虑的元素，证明了两种方法获得的数据之间的强关联性（R 2

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