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首页> 美国卫生研究院文献>other >Quantification of Mg2+ Ca2+ and H+ transport by the gastrointestinal tract of the goldfish Carassius auratus using the Scanning Ion-selective Electrode Technique (SIET)
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Quantification of Mg2+ Ca2+ and H+ transport by the gastrointestinal tract of the goldfish Carassius auratus using the Scanning Ion-selective Electrode Technique (SIET)

机译：使用扫描离子选择电极技术（SIET）对金鱼Car鱼胃肠道中Mg2 +Ca2 +和H +的转运进行定量

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An in vitro gut-sac technique and the scanning ion-selective electrode technique (SIET) were used to characterize Mg2+, Ca2+, and H+ transport at both the mucosal and serosal surfaces of non-everted and everted gastrointestinal tissues obtained from Carassius auratus. As part of the study, two magnesium ionophores were compared (II vs. VI). Unfed animals displayed uniform transport of all ions along the intestine. Feeding resulted in elevated Mg2+ and Ca2+ transport when the gut lumen contained chyme however, under symmetrical conditions this increased transport rate was absent. Furthermore, zonation of divalent cation transport was present for both Ca2+ and Mg2+ under non-symmetrical conditions while the zonation remained for Ca2+ alone under symmetrical conditions. High dietary Mg2+ decreased absorption and induced secretion of Mg2+ in the posterior intestine. Uptake kinetics in the esophagus suggest large diffusive and/or convective components based on a linear relationship between Mg2+ transport and concentration and lack of inhibition by ouabain, an inhibitor of Na+-K+-ATPase. In contrast, kinetics in the rectum were suggestive of a low affinity, saturable carrier-mediated pathway. A decrease in Mg2+ and Ca2+ transport was observed in the posterior intestine (both at the mucosal and serosal surfaces) in response to ouabain. This impact was greatest for Ca2+ transport and when applied to the mucosal fluid and measured in everted preparations. In contrast a putative Mg2+ transport inhibitor, cobalt(III)hexamine-chloride, did not affect Mg2+ transport. This is the first study to use SIET approaches to study ion transport in the gut of teleost fish. This is also the first study to provide characterization of Mg2+ transport in the gut of C. auratus. Due to the limited selectivity of Magnesium ionophore II, subsequent studies of tissues bathed in physiological saline should be made using Magnesium Ionophore VI.
机译：利用体外肠囊技术和扫描离子选择电极技术（SIET）表征Mg 2 + ，Ca 2 + 和H + 从Car鱼的非外翻和外翻胃肠组织的粘膜和浆膜表面运输。作为研究的一部分，比较了两个镁离子载体（II与VI）。未进食的动物在肠道中显示出所有离子的均匀传输。当肠腔中含有食糜时，饲喂导致Mg 2 + 和Ca 2 + 运输增加，但是在对称条件下，运输速度却没有增加。此外，在非对称条件下，Ca 2 + 和Mg 2 + 均存在二价阳离子迁移带，而Ca 2 + 在对称条件下单独使用。饮食中高含量的Mg 2 + 会降低后肠Mg 2 + 的吸收并诱导其分泌。根据Mg 2 + 转运与浓度之间的线性关系，食管中的摄取动力学表明存在较大的扩散和/或对流成分，而哇巴因（Na + -K + -ATPase。相反，直肠中的动力学提示低亲和力，可饱和的载体介导的途径。响应于哇巴因，在后肠（粘膜和浆膜表面）Mg 2 + 和Ca 2 + 的运输减少。这种影响对于Ca 2 + 的运输是最大的，当应用于粘膜液并在外翻制剂中进行测量时，影响最大。相反，假定的Mg 2 + 传输抑制剂，氯化钴（III）六胺不影响Mg 2 + 传输。这是第一项使用SIET方法研究硬骨鱼肠中离子迁移的研究。这也是第一个提供表征金黄色葡萄球菌肠中Mg 2 + 转运的研究。由于镁离子载体II的选择性有限，因此应使用镁离子载体VI对随后沐浴在生理盐水中的组织进行研究。

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