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首页> 美国卫生研究院文献>other >Purification and Partial Characterization of Bacteriocin Lac-B23 a Novel Bacteriocin Production by Lactobacillus plantarum J23 Isolated From Chinese Traditional Fermented Milk
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Purification and Partial Characterization of Bacteriocin Lac-B23 a Novel Bacteriocin Production by Lactobacillus plantarum J23 Isolated From Chinese Traditional Fermented Milk

机译：分离自中国传统发酵乳的植物乳杆菌J23生产的新型细菌素细菌素Lac-B23的纯化和部分表征

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摘要

The exploration and evaluation of bacteriocin-producing lactic acid bacteria (LAB) have been one of the powerful means to food preservation. A total of 300 strains were isolated from Chinese traditional fermented milk products. A bacteriocin-producing LAB, named Lactobacillus plantarum J23, was screened and identified. Bacteriocin Lac-B23 from L. plantarum J23 was purified by 80% ammonium sulfate precipitation, cation-exchange chromatography, and reverse-phase high-performance liquid chromatography. Molecular weight of bacteriocin Lac-B23 was determined to be approximately 6.73 kDa by tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, and it was confirmed as a novel bacteriocin by liquid chromatography-mass spectrometry. Moreover, bacteriocin Lac-B23 showed thermal stability when heated at below 100°C for 30 min, pH stability between pH 2.0 and 12.0, and sensitivity to trypsin, proteinase K, and proteinase E. The antimicrobial activity of bacteriocin could be enhanced by addition of Fe2+, Mn2+, and ethyl alcohol, and inhibited by Cu2+, K+, Ca2+, Zn2+, Mg2+, and sodium chloride. The results suggested bacteriocin Lac-B23 to have potential application prospects in the food industry.
机译：产生细菌素的乳酸菌（LAB）的探索和评估已成为食品保存的有力手段之一。从中国传统发酵乳制品中分离出总共300株。筛选并鉴定了产生细菌素的实验室，称为植物乳杆菌J23。通过80％硫酸铵沉淀，阳离子交换色谱和反相高效液相色谱纯化来自植物乳杆菌J23的细菌素Lac-B23。通过三十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析确定细菌素Lac-B23的分子量为约6.73kDa，并且通过液相色谱-质谱法确认为新型细菌素。此外，细菌素Lac-B23在低于100°C的温度下加热30分钟时表现出热稳定性，pH稳定性在pH 2.0和12.0之间，并且对胰蛋白酶，蛋白酶K和蛋白酶E敏感。通过添加细菌，可以增强细菌素的抗菌活性。 Fe 2 + ，Mn 2 + 和乙醇的含量，并被Cu 2 + ，K + 抑制，Ca 2 + ，Zn 2 + ，Mg 2 + 和氯化钠。结果表明细菌素Lac-B23在食品工业中具有潜在的应用前景。

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