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Expression of Tim-3 in peripheral blood mononuclear cells and placental tissue in unexplained recurrent spontaneous abortion

机译：不明原因反复自然流产患者外周血单个核细胞和胎盘组织中Tim-3的表达

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摘要

The expression of T-cell immunoglobulin domain, mucin domain-3 (Tim-3) in unexplained recurrent spontaneous abortion (URSA) was investigated.Tim-3 mRNA expression in peripheral blood mononuclear cells (PBMCs) of URSA and control groups was assayed by fluorescent quantitative real-time polymerase chain reaction. Tim-3 protein expression intensity and localization in placental villi and uterine decidua were determined using immunohistochemical assay. The CD4+Tim-3+/CD4+ cell ratio in PBMCs was determined by flow cytometry.Tim-3 mRNA expression in PBMCs was significantly higher in URSA than in normal controls (1.32 ± 0.25 vs 1.20 ± 0.12, P < .05). Tim-3 was expressed in placental tissue from both URSA patients and normal pregnant females (controls); however, the expression intensity was higher in the URSA group (0.54 ± 0.31 vs 0.35 ± 0.22, P < .05). CD4+Tim-3+/CD4+ cell ratio in PBMCs was significantly higher in the URSA group than that in the control group (4.53 ± 1.66% vs 1.28 ± 0.71%, P < .05).Increased Tim-3 expression in PBMCs and placental tissue of URSA might affect maternal-fetal immune tolerance. Tim-3 was involved in the pathogenesis of URSA, which was expected to serve as an indicator for the immune evaluation of URSA.
机译：研究了原因不明的反复自然流产（URSA）中T细胞免疫球蛋白结构域，粘蛋白结构域3（Tim-3）的表达，并通过URSA和对照组检测了外周血单个核细胞（PBMC）中Tim-3 mRNA的表达。荧光定量实时聚合酶链反应。使用免疫组织化学方法确定Tim-3蛋白的表达强度和在胎盘绒毛和子宫蜕膜中的定位。流式细胞仪检测PBMC中CD4 + Tim-3 + / CD4 + 细胞比例，PBMC中Tim-3 mRNA的表达显着URSA比正常对照组高（1.32±±0.25 vs 1.20±0.12，P <0.05）。 Tim-3在URSA患者和正常妊娠女性（对照组）的胎盘组织中表达。然而，URSA组的表达强度更高（0.54±0.31 vs 0.35±0.22，P <0.05）。 URSA组的PBMC中CD4 + Tim-3 + / CD4 + 的细胞比率显着高于对照组（4.53± 1.66％vs 1.28％±0.71％，P <.05）。URMCPBMCs和胎盘组织中Tim-3表达的升高可能影响母胎免疫耐受。 Tim-3参与了URSA的发病机制，有望作为URSA免疫评估的指标。

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