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首页> 美国卫生研究院文献>other >Biocontrol Rhizobacterium Pseudomonas sp. 23S Induces Systemic Resistance in Tomato (Solanum lycopersicum L.) Against Bacterial Canker Clavibacter michiganensis subsp. michiganensis
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Biocontrol Rhizobacterium Pseudomonas sp. 23S Induces Systemic Resistance in Tomato (Solanum lycopersicum L.) Against Bacterial Canker Clavibacter michiganensis subsp. michiganensis

机译：生物防治假单胞菌假单胞菌23S诱导番茄（Solanum lycopersicum L.）对细菌性溃疡病密歇根州立细菌亚种的系统抗性。密歇根州

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Tomato bacterial canker disease, caused by Clavibacter michiganensis subsp. michiganensis (Cmm) is a destructive disease and has been a serious concern for tomato industries worldwide. Previously, a rhizosphere isolated strain of Pseudomonas sp. 23S showed antagonistic activity toward Cmm in vitro. This Pseudomonas sp. 23S was characterized to explore the potential of this bacterium for its use in agriculture. Pseudomonas sp. 23S possesses ability to solubilize inorganic phosphorus, and to produce siderophores, indole acetic acid, and hydrogen cyanide. The strain also showed antagonistic activity against Pseudomonas syringae pv. tomato DC 3000. A plant assay indicated that Pseudomonas sp. 23S could promote growth of tomato seedlings. The potential of treating tomato plants with Pseudomonas sp. 23S to reduce the severity of tomato bacterial canker by inducing systemic resistance (ISR) was investigated using well characterized marker genes such as PR1a [salicylic acid (SA)], PI2 [jasmonic acid (JA)], and ACO [ethylene (ET)]. Two-week-old tomato plants were treated with Pseudomonas sp. 23S by soil drench, and Cmm was inoculated into the stem by needle injection on 3, 5, or 7 days post drench. The results indicated that plants treated with Pseudomonas sp. 23S, 5 days prior to Cmm inoculation significantly delayed the progression of the disease. These plants, after 3 weeks from the date of Cmm inoculation, had significantly higher dry shoot and root weight, higher levels of carbon, nitrogen, phosphorus, and potassium in the leaf tissue, and the number of Cmm population in the stem was significantly lower for the plants treated with Pseudomonas sp. 23S. From the real-time quantitative PCR (qRT-PCR) analysis, the treatment with Pseudomonas sp. 23S alone was found to trigger a significant increase in the level of PR1a transcripts in tomato plants. When the plants were treated with Pseudomonas sp. 23S and inoculated with Cmm, the level of PR1a and ACO transcripts were increased, and this response was faster and greater as compared to plants inoculated with Cmm but not treated with Pseudomonas sp. 23S. Overall, the results suggested the involvement of SA signaling pathways for ISR induced by Pseudomonas sp. 23S.
机译：密歇根州细枝杆菌亚种引起的番茄细菌性溃疡病。密歇根州（Cmm）是一种破坏性疾病，已成为全世界番茄行业的严重关注点。以前，根际分离的假单胞菌菌种。 23S显示出对Cmm的体外拮抗活性。这假单胞菌属。 23S的特点是探索这种细菌在农业中的应用潜力。假单胞菌23S具有溶解无机磷，产生铁载体，吲哚乙酸和氰化氢的能力。该菌株还显示出对丁香假单胞菌PV的拮抗活性。番茄DC3000。植物测定表明假单胞菌属。 23S可以促进番茄幼苗的生长。用假单胞菌处理番茄植物的潜力。使用特征明确的标记基因，例如PR1a [水杨酸（SA）]，PI2 [茉莉酸（JA）]和ACO [乙烯（ET）），研究了通过诱导系统抗性（ISR）降低番茄细菌​​性溃疡病严重程度的23S。 ]。用假单胞菌属（Pseudomonas sp。）处理两周大的番茄植物。在土壤中淋洗23S，在淋水后3、5或7天通过针头注射将Cmm接种到茎中。结果表明用假单胞菌sp 处理过的植物。接种Cem 前5天的23S明显延迟了疾病的进展。这些植物在接种 Cmm 后3周后，干梢和根重显着提高，叶片组织中的碳，氮，磷和钾含量更高，并且<用假单胞菌属 23S处理过的植物茎中的em> Cmm 种群显着降低。通过实时定量PCR（qRT-PCR）分析，发现单独使用假单胞菌（Pseudomonas sp。） 23S处理可触发 PR1a 转录本水平的显着增加。番茄植物。用假单胞菌sp。 23S处理植物并接种 Cmm 后， PR1a 和 ACO 转录本的水平与接种 Cmm 但未接种 Pseudomonas sp。 23S的植物相比，该反应更快，更强。总体而言，结果表明假单胞菌 23S诱导的ISR的SA信号通路参与其中。

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