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Plate Design for and Cherry Picking of Bacterial RNAi Clones for Systematic Error Detection in High-Throughput C. elegans RNAi Screens

机译:用于高通量线虫RNAi筛选系统性错误检测的细菌RNAi克隆的平板设计和Cherry采摘

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摘要

Automated or semi-automated high-throughput RNAi screens are highly prone to systematic errors because of multistep repetitive protocols and extensive use of automated instruments. A well-designed RNAi library can help detect and minimize systematic human and robotic errors. In this Unit, we described how to design an RNAi bacterial library used in conjunction with the well-studied nematode Caenorhabditis elegans for automated phenotypic screens. We provide strategies to design and assemble RNAi libraries to reduce or eliminate systematic errors. These strategies serve as a good quality control (QC) check and facilitate obtaining high-quality data in a genome-wide and sub-library RNAi screen.
机译:自动化或半自动化的高通量RNAi筛查极易发生系统错误,这是因为多步骤重复操作规程和自动化仪器的广泛使用。精心设计的RNAi文库可以帮助检测并最大程度地减少系统性的人为和机器人错误。在本单元中,我们描述了如何设计与经过充分研究的线虫秀丽隐杆线虫一起使用的RNAi细菌文库,用于自动表型筛选。我们提供设计和组装RNAi文库的策略,以减少或消除系统错误。这些策略可作为良好的质量控制(QC)检查,并有助于在全基因组和亚库RNAi筛选中获得高质量数据。

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