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首页> 美国卫生研究院文献>other >Overexpression of SSBXoc a Single-Stranded DNA-Binding Protein From Xanthomonas oryzae pv. oryzicola Enhances Plant Growth and Disease and Salt Stress Tolerance in Transgenic Nicotiana benthamiana
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Overexpression of SSBXoc a Single-Stranded DNA-Binding Protein From Xanthomonas oryzae pv. oryzicola Enhances Plant Growth and Disease and Salt Stress Tolerance in Transgenic Nicotiana benthamiana

机译：SSBXoc的过表达米黄单胞菌PV中的单链DNA结合蛋白。稻提高转基因烟草本生烟草的植物生长和抗盐胁迫能力

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We previously reported that SSBXoc, a highly conserved single-stranded DNA-binding protein from Xanthomonas spp., was secreted through the type III secretion system (T3SS) and functioned as a harpin-like protein to elicit the hypersensitive response (HR) in the non-host plant, tobacco. In this study, we cloned SsbXoc gene from X. oryzae pv. oryzicola (Xoc), the causal agent of bacterial leaf streak in rice, and transferred it into Nicotiana benthamiana via Agrobacterium-mediated transformation. The expression of SsbXoc in transgenic N. benthamiana enhanced growth of both seedling and adult plants. When inoculated with the harpin Hpa1 or the pathogen Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), the accumulation of reactive oxygen species (ROS) was increased more in SsbXoc transgenic lines than that in wild-type (WT) plants. The expression of pathogenesis-related protein genes (PR1a and SGT1), HR marker genes (HIN1 and HSR203J) and the mitogen-activated protein kinase pathway gene, MPK3, was significantly higher in transgenic lines than in WT after inoculation with Pst DC3000. In addition, SsbXoc transgenic lines showed the enhanced resistance to the pathogenic bacteria P. s. tabaci and the improved tolerance to salt stress, accompanied by the elevated transcription levels of the defense- and stress-related genes. Taken together, these results indicate that overexpression of the SsbXoc gene in N. benthamiana significantly enhanced plant growth and increased tolerance to disease and salt stress via modulating the expression of the related genes, thus providing an alternative approach for development of plants with improved tolerance against biotic and abiotic stresses.
机译：我们先前曾报道SSBXoc是Xanthomonas spp。的一种高度保守的单链DNA结合蛋白，是通过III型分泌系统（T3SS）分泌的，并起着类似harpin的蛋白的作用，从而引起了超敏反应（HR）。非寄主植物，烟草。在这项研究中，我们从米曲霉PV中克隆了SsbXoc基因。稻中细菌叶条纹的病原体稻（Oryzicola，Xoc）通过农杆菌介导的转化将其转移到本氏烟草中。 SsbXoc在转基因本氏烟草中的表达增强了幼苗和成年植物的生长。当用harpin Hpa1或病原体丁香假单胞菌pv接种时。番茄DC3000（Pst DC3000）中，与野生型（WT）植物相比，SsbXoc转基因品系中活性氧（ROS）的积累更多。发病相关蛋白基因（PR1a和SGT1），HR标记基因（HIN1和 HSR203J ）和丝裂原激活的蛋白激酶途径基因 MPK3 的表达显着 Pst DC3000接种后，转基因品系比野生型更高。此外， SsbXoc 转基因品系对病原菌 P 的抗性增强。 s 。 tabaci 和对盐胁迫的增强耐受性，同时伴随着防御和胁迫相关基因的转录水平升高。综上，这些结果表明 N 中 SsbXoc 基因的过表达。 benthamiana 通过调节相关基因的表达，显着增强了植物的生长并增强了对疾病和盐胁迫的耐受性，从而为开发具有更高的对生物和非生物胁迫耐受性的植物提供了另一种方法。

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