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A rapid and simple chemical method for the preparation of Ag colloids for surface-enhanced Raman spectroscopy using the Ag mirror reaction

机译：一种快速简单的化学方法利用银镜反应制备用于表面增强拉曼光谱的银胶体

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摘要

Colloidal silver (Ag) nanoparticles (AgNP) have been widely used for surface-enhanced Raman spectroscopy (SERS) applications. We report a simple, rapid and effective method to prepare AgNP colloids for SERS using the classic organic chemistry Ag mirror reaction with Tollens’ reagent. The AgNP colloid prepared with this process was characterized using SEM, and the reaction conditions further optimized using SERS measurements. It was found that Ag mirror reaction conditions that included 20 mM AgNO3, 5 min reaction time, and 0.5 M glucose produced AgNP colloids with an average size of 319.1 nm (s.d ±128.1). These AgNP colloids exhibited a significant SERS response when adenine was used as the reporter molecule. The usefulness of these new AgNP colloids was demonstrated by detecting the nucleotides adenosine 5′-monophosphate (AMP), guanosine 5′-monophosphate (GMP), cytidine 5′-monophosphate (CMP), and uridine 5′-monophosphate (UMP). A detection limit of 500 nM for AMP was achieved with the as-prepared AgNP colloid. The bacterium Mycoplasma pneumoniae was also easily detected in laboratory culture with these SERS substrates. These findings attest to the applicability of this AgNP colloid for the sensitive and specific detection of both small biomolecules and microorganisms.
机译：胶体银（Ag）纳米粒子（AgNP）已被广泛用于表面增强拉曼光谱（SERS）应用。我们报告了一种简单，快速且有效的方法，该方法使用经典的有机化学银镜反应与Tollens试剂制备SERS的AgNP胶体。用SEM表征了用该方法制备的AgNP胶体，并通过SERS测量进一步优化了反应条件。发现包括20 mM AgNO3、5分钟反应时间和0.5 M葡萄糖的Ag镜面反应条件产生的AgNP胶体的平均大小为319.1 nm（标准偏差±128.1）。当腺嘌呤用作报告分子时，这些AgNP胶体表现出明显的SERS反应。通过检测核苷酸5'-单磷酸（AMP），鸟苷5'-单磷酸（GMP），胞苷5'-单磷酸（CMP）和尿苷5'-单磷酸（UMP）核苷酸证明了这些新型AgNP胶体的有用性。制备的AgNP胶体对AMP的检出限为500 nM。使用这些SERS底物在实验室培养中也很容易检测到肺炎支原体细菌。这些发现证明了这种AgNP胶体适用于小型生物分子和微生物的灵敏和特异检测。

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