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Development of Colloidal Gold-Based Immunochromatographic Assay for Rapid Detection of Goose Parvovirus

机译：快速检测鹅细小病毒的胶体金基免疫色谱分析方法的建立

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Goose parvovirus (GPV) remains as a worldwide problem in goose industry. For this reason, it is necessary to develop a new diagnostic approach that is easier and faster than conventional tests. A rapid immunochromatographic assay based on antibody colloidal gold nanoparticles specific to GPV was developed for the detection of GPV in goose allantoic fluid and supernatant of tissue homogenate. The monoclonal antibodies (Mab) was produced by immunizing the BALB/c mice with purified GPV suspension, and the polyclonal antibody (pAb) was produced by immunizing the rabbits with recombinant VP3 protein. The colloidal gold was prepared by the reduction of gold salt with sodium citrate coupled with Mab against GPV. The optimal concentrations of the coating antibody and capture antibody were determined to be 1.6 mg/ml and 9 μg/ml. With visual observation, the lower limit was found to be around 1.2 μg/ml. Common diseases of goose were tested to evaluate the specificity of the immune colloidal gold (ICG) strip, and no cross-reaction was observed. The clinical detection was examined by carrying out the ICG strip test with 92 samples and comparing the results of these tests with those obtained via agar diffusion test and polymerase chain reaction (PCR) test. Therefore, the ICG strip test was a sufficiently sensitive and accurate detection method for a rapid screening of GPV.
机译：鹅细小病毒（GPV）仍然是鹅业的全球性问题。因此，有必要开发一种比常规测试更容易，更快捷的新诊断方法。建立了基于对GPV特异性的抗体胶体金纳米颗粒的快速免疫色谱分析方法，用于检测鹅尿囊液和组织匀浆上清液中的GPV。通过用纯化的GPV悬浮液免疫BALB / c小鼠来产生单克隆抗体（Mab），并通过用重组VP3蛋白免疫兔子来产生多克隆抗体（pAb）。胶体金是通过用柠檬酸钠和Mab还原GPV还原金盐而制得的。确定包被抗体和捕获抗体的最佳浓度为1.6 mg / ml和9μg/ ml。通过目视观察，发现下限为约1.2μg/ ml。测试了鹅的常见疾病，以评估免疫胶体金（ICG）条的特异性，未观察到交叉反应。通过对92个样品进行ICG剥离测试并将这些测试的结果与通过琼脂扩散测试和聚合酶链反应（PCR）测试获得的结果进行比较，来检查临床检测。因此，ICG剥离测试是用于快速筛选GPV的足够灵敏且准确的检测方法。

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