首页> 美国卫生研究院文献>other >A Method for Selective Depletion of Zn(II) Ions from Complex Biological Media and Evaluation of Cellular Consequences of Zn(II) Deficiency

【2h】

A Method for Selective Depletion of Zn(II) Ions from Complex Biological Media and Evaluation of Cellular Consequences of Zn(II) Deficiency

机译：一种从复杂生物介质中选择性去除锌离子的方法和细胞缺锌症的评估

代理获取

本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。

页面导航

摘要
著录项
相似文献
相关主题

摘要

We describe the preparation, evaluation, and application of an S100A12 protein-conjugated solid support, hereafter the “A12-resin,” that can remove 99% of Zn(II) from complex biological solutions without significantly perturbing the concentrations of other metal ions. The A12-resin can be applied to selectively deplete Zn(II) from diverse tissue culture media and from other biological fluids, including human serum. To further demonstrate the utility of this approach, we investigated metabolic, transcriptomic, and metallomic responses of HEK293 cells cultured in medium depleted of Zn(II) using S100A12. The resulting data provide insight into how cells respond to acute Zn(II) deficiency. We expect that the A12-resin will facilitate interrogation of disrupted Zn(II) homeostasis in biological settings, uncovering novel roles for Zn(II) in biology.

机译：我们描述了S100A12蛋白缀合的固体支持物（以下称为“ A12树脂”）的制备，评估和应用，该支持物可从复杂的生物溶液中去除99％的Zn（II），而不会显着干扰其他金属离子的浓度。可以将A12树脂用于从各种组织培养基和包括人血清在内的其他生物流体中选择性地消耗Zn（II）。为了进一步证明该方法的实用性，我们研究了使用S100A12在缺锌（II）培养基中培养的HEK293细胞的代谢，转录组和金属组学响应。所得数据可提供有关细胞如何应对急性Zn（II）缺乏症的见解。我们期望，A12树脂将有助于在生物环境中询问被破坏的Zn（II）稳态，从而揭示Zn（II）在生物学中的新作用。

著录项

期刊名称 other
作者
Christopher E. R. Richardson; Lisa S. Cunden; Vincent L. Butty; Elizabeth M. Nolan; Stephen J. Lippard; Matthew D. Shoulders;
展开▼
作者单位

展开▼
年(卷),期 -1(140),7
年度 -1
页码 2413–2416
总页数 8
原文格式 PDF
正文语种
中图分类
关键词

相似文献

外文文献
中文文献
专利

1. A Method for Selective Depletion of Zn(Ⅱ) Ions from Complex Biological Media and Evaluation of Cellular Consequences of Zn(Ⅱ) Deficiency [J] . Christopher E. R. Richardson, Lisa S. Cunden, Vincent L. Butty, Journal of the American Chemical Society . 2018,第7期

机译：一种从复杂生物介质中选择性去除锌离子的方法及细胞缺锌症的评估
2. Synthesis, spectral and structural characterization of Ni(II), Cu(II), Zn(II), Cd(II) and Hg(II) complexes with 2-mercapto-5-methyl-1,3,4-thiadiazole: A Zn(II) complex acting as a new sensitive and selective fluorescent probe for the detection of Hg~(2+) in H_2O-MeOH medium [J] . Bharati P., Bharti A., Bharty M.K., Polyhedron: The International Journal for Inorganic and Organometallic Chemistry . 2013,第Null期

机译：Ni（II），Cu（II），Zn（II），Cd（II）和Hg（II）与2-巯基-5-甲基-1,3,4-噻二唑的配合物的合成，光谱和结构表征Zn（II）配合物用作检测H_2O-MeOH介质中Hg〜（2+）的新型灵敏选择性荧光探针
3. Synthesis, spectral characterization and biological evaluation of Mn(II), Co(II), Ni(II), Cu(II), Zn(II) and Cd(II) complexes with thiosemicarbazone ending by pyrazole and pyridyl rings [J] . T.A. Yousef, G.M. Abu El-Reash, M. Al-Jahdali, Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy . 2014,第Null期

机译：Mn（II），Co（II），Ni（II），Cu（II），Zn（II）和Cd（II）与以吡唑和吡啶环为环的硫代半脲络合物的合成，光谱表征和生物学评价
4. Fabrication of the nanoparticles of the Cu(II), Zn(II) complexes with pyrithione derivative and their marine biological activities [C] . Wen-Ge Li, Yan An, Xiao-Feng Li, PFAM XX . 2012

机译：用吡啶硫代硫代衍生物及其海洋生物活性制备Cu（II），Zn（II）复合物的纳米颗粒
5. A Fluorescence Quenching Study of the Human Serum Albumin-Quercetin Complex by Addition of Cd(II), Zn(II), and Co(II) [D] . Okorafor, Udochukwu 2015

机译：加入Cd（II），Zn（II）和Co（II）的人血清白蛋白-槲皮素复合物的荧光猝灭研究
6. Synthesis Characterization and Biological Evaluation of Co(II) Cu(II) Ni(II) and Zn(II) Complexes With Cephradine [O] . Zahid H. Chohan, Maimoon F. Jaffery 2000

机译：头孢拉定Co（II）Cu（II）Ni（II）和Zn（II）配合物的合成表征和生物学评价
7. A Method for Selective Depletion of Zn(II) Ions from Complex Biological Media and Evaluation of Cellular Consequences of Zn(II) Deficiency [O] . Christopher E. R. Richardson, Lisa S. Cunden, Vincent L. Butty, 2018

机译：一种从复杂生物培养基中选择性耗竭Zn（II）离子的方法及Zn（II）缺乏的胞质后果评价

A Method for Selective Depletion of Zn(II) Ions from Complex Biological Media and Evaluation of Cellular Consequences of Zn(II) Deficiency

摘要

著录项

相似文献

相关主题

期刊订阅