• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>other >Long-lasting Corolla Cultivars in Japanese Azaleas: A Mutant AP3/DEF Homolog Identified in Traditional Azalea Cultivars from More Than 300 Years Ago
【2h】

Long-lasting Corolla Cultivars in Japanese Azaleas: A Mutant AP3/DEF Homolog Identified in Traditional Azalea Cultivars from More Than 300 Years Ago

机译：日本杜鹃花中的持久花冠品种：300多年以前的传统杜鹃花品种中鉴定出突变的AP3 / DEF同源物

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Floral shape in higher plants typically requires genetic regulation through MADS transcription factors. In Japan, hundreds of azalea cultivars including flower shape mutations have been selected from the diversity of endogenous species and natural hybrids since the early 17th century, the Edo era (1603–1867). The long-lasting trait, known as “Misome-shō” in Japanese, has been identified in several species and cultivar groups of evergreen azaleas (Rhododendron L.) from three hundred years ago in Japan. However, the natural mutation conferring the long-lasting trait in azalea remains unknown. Here, we showed MADS-box gene mutations in long-lasting flowers, R. kaempferi ‘Nikkō-misome,’ R. macrosepalum ‘Kochō-zoroi,’ R. indicum ‘Chōjyu-hō,’ and R. × hannoense ‘Amagi-beni-chōjyu.’ All of the long-lasting flowers exhibited small-sized corollas with stomata during long blooming. In the long-lasting flowers, transcript of the APETALA3 (AP3)/DEFICIENS (DEF) homolog was reduced, and an LTR-retrotransposon was independently inserted into exons 1, 2, and 7 or an unknown sequence in exon 1 in gDNA of each cultivar. This insertion apparently abolished the normal mRNA sequence of the AP3/DEF homolog in long-lasting flowers. Also, long-lasting flowers were shown from F2 hybrids that had homozygous ap3/def alleles. Therefore, we concluded that the loss of function of the AP3/DEF homolog through a transposable element insertion may confer a stable long-lasting mutation in evergreen azaleas.
机译：高等植物的花型通常需要通过MADS转录因子进行遗传调控。在日本，自17世纪初期（江户时代（1603-1867年））以来，已经从内源物种和自然杂种的多样性中选出了数百个包括花朵形状突变在内的杜鹃花品种。日本人在300年前就已经在几种常绿杜鹃花（Rhododendron L.）的物种和栽培品种组中发现了这种长久的性状，在日语中被称为“Misome-shō”。然而，赋予杜鹃花持久性状的自然突变仍然未知。在这里，我们显示了长花，R。kaempferi'Nikkō-misome，R。macrosepalum'Kochō-zoroi'，R。indicum'Chōjyu-hō和R.×hannoense'Amagi- beni-chōjyu。'所有长久开花的花朵在长期开花时都呈现出带有气孔的小花冠。在长寿花中，APETALA3（AP3）/ DEFICIENS（DEF）同源物的转录物减少，并且将LTR-逆转座子独立插入每个外显子1、2和7或外显子1的gDNA的未知序列中品种这种插入显然消除了长寿花中AP3 / DEF同源物的正常mRNA序列。同样，显示了具有纯合的ap3 / def等位基因的F2杂种的持久花。因此，我们得出的结论是，通过转座因子插入而导致AP3 / DEF同源物功能丧失可能会在常绿杜鹃花中产生稳定的持久突变。

著录项

相似文献

  • 外文文献
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号