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Flexible wide-field optical micro-angiography based on Fourier-domain multiplexed dual-beam swept source optical coherence tomography

机译:基于傅里叶域多路复用双束扫频源光学相干断层扫描的柔性广域光学微血管造影

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摘要

Wide-field optical coherence tomography angiography (OCTA) is gaining interest in clinical imaging applications. In this pursuit, it is challenging to maintain the imaging resolution and sensitivity throughout the wide field of view (FoV). Here, we propose a novel method/system of dual-beam arrangement and Fourier domain multiplexing to achieve wide field OCTA when imaging the uneven surface samples. The proposed system provides two separate FoVs, with flexibility that the imaging area, focus of the imaging beam and imaging depth range can be individually adjusted for each FoV, leading to either 1) increased system imaging FoV or 2) capability of targeting two regions of interests that locate at depths with large difference between each other. We demonstrate this novel method by employing 100 kHz laser source in a swept source OCTA to achieve an effective 200 kHz sweeping rate, covering a 22 × 22 mm FoV. The results are verified by a SS-OCTA system employing a 200 kHz laser source, together with the experimental demonstrations when imaging whole brain vasculature in rodent models and skin blood perfusion in human fingers, show-casing the capability of proposed system to image live large samples with complex surface topography.
机译:广域光学相干断层扫描血管造影(OCTA)在临床成像应用中引起了人们的兴趣。在这种追求中,在整个宽视场(FoV)中保持成像分辨率和灵敏度都具有挑战性。在这里,我们提出了一种新的方法/系统的双光束安排和傅立叶域复用,以实现对不平坦的表面样本成像时的宽场OCTA。拟议的系统提供两个单独的FoV,具有灵活性,可以针对每个FoV分别调整成像区域,成像光束的聚焦和成像深度范围,从而导致1)增加系统成像FoV或2)瞄准两个区域的能力。位于彼此之间差异很大的深度的兴趣。我们通过在扫频源OCTA中采用100 kHz激光源来演示这一新颖方法,以实现有效的200 kHz扫频速率,覆盖22×22 mm FoV。通过使用200 kHz激光源的SS-OCTA系统,以及在啮齿动物模型中对全脑血管成像和人手指的皮肤血流灌注成像的实验演示,对结果进行了验证,这证明了拟议系统对大型活体成像的能力具有复杂表面形貌的样品。

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