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首页> 美国卫生研究院文献>Journal of Genomics >SELfies and CELLfies: Whole Genome Sequencing and Annotation of Five Antibiotic Resistant Bacteria Isolated from the Surfaces of Smartphones An Inquiry Based Laboratory Exercise in a Genomics Undergraduate Course at the Rochester Institute of Technology
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SELfies and CELLfies: Whole Genome Sequencing and Annotation of Five Antibiotic Resistant Bacteria Isolated from the Surfaces of Smartphones An Inquiry Based Laboratory Exercise in a Genomics Undergraduate Course at the Rochester Institute of Technology

机译：自我和细胞：从智能手机表面分离的五种抗药性细菌的全基因组测序和注释罗彻斯特理工学院基因组学本科课程的基于研究的实验练习

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摘要

Are touchscreen devices a public health risk for the transmission of pathogenic bacteria, especially those that are resistant to antibiotics? To investigate this, we embarked on a project aimed at isolating and identifying bacteria that are resistant to antibiotics from the screens of smartphones. Touchscreen devices have become ubiquitous in society, and it is important to evaluate the potential risks they pose towards public health, especially as it pertains to the harboring and transmission of pathogenic bacteria that are resistant to antibiotics. Sixteen bacteria were initially isolated of which five were unique (four Staphylococcus species and one Micrococcus species). The genomes of the five unique isolates were subsequently sequenced and annotated. The genomes were analyzed using in silico tools to predict the synthesis of antibiotics and secondary metabolites using the >antibiotics and >Secondary >Metabolite >Analysis >SHell (antiSMASH) tool in addition to the presence of gene clusters that denote resistance to antibiotics using the >Resistance >Gene >Identifier (RGI) tool. In vivo analysis was also done to assess resistance/susceptibility to four antibiotics that are commonly used in a research laboratory setting. The data presented in this manuscript is the result of a semester-long inquiry based laboratory exercise in the genomics course (BIOL340) in the Thomas H. Gosnell School of Life Sciences/College of Science at the Rochester Institute of Technology.
机译：触摸屏设备是否对传播病原细菌（特别是对抗生素具有抵抗力的细菌）具有公共健康风险？为了对此进行调查，我们启动了一个旨在从智能手机屏幕中分离和鉴定对抗生素具有抗性的细菌的项目。触摸屏设备已在社会中变得无处不在，并且评估它们对公共健康构成的潜在风险非常重要，尤其是因为它涉及对抗生素具有抗药性的病原菌的藏匿和传播。最初分离出16种细菌，其中5种是独特的（4种葡萄球菌和1种微球菌）。随后对这五个独特分离株的基因组进行了测序和注释。使用计算机软件对基因组进行了分析，以预测使用>抗生物和> S 杂菌> M etabolite >的抗生素和次生代谢产物的合成除了使用> R 抗性> G 表示对抗生素具有抗药性的基因簇之外，还提供了一种分析> SH ell（antiSMASH）工具。 > ene > I 标识符（RGI）工具。还进行了体内分析，以评估对研究实验室环境中常用的四种抗生素的耐药性/敏感性。该手稿中的数据是罗切斯特理工学院生命科学学院/科学学院Thomas H. Gosnell生命科学学院的基因组学课程（BIOL340）中基于研究期的研究活动的结果。

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