• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>other >Molecular markers in keratins from Mysticeti whales for species identification of baleen in museum and archaeological collections
【2h】

Molecular markers in keratins from Mysticeti whales for species identification of baleen in museum and archaeological collections

机译：Mysticeti鲸的角蛋白中的分子标记可用于博物馆和考古收藏中的巴里翁物种鉴定

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Baleen has been harvested by indigenous people for thousands of years, as well as collected by whalers as an additional product of commercial whaling in modern times. Baleen refers to the food-filtering system of Mysticeti whales; a full baleen rack consists of dozens of plates of a tough and flexible keratinous material that terminate in bristles. Due to its properties, baleen was a valuable raw material used in a wide range of artefacts, from implements to clothing. Baleen is not widely used today, however, analyses of this biomolecular tissue have the potential to contribute to conservation efforts, studies of genetic diversity and a better understanding of the exploitation and use of Mysticeti whales in past and recent times. Fortunately, baleen is present in abundance in museum natural history collections. However, it is often difficult or impossible to make a species identification of manufactured or old baleen. Here, we propose a new tool for biomolecular identification of baleen based on its main structural component alpha-keratin (the same protein that makes up hair and fingernails). With the exception of minke whales, alpha-keratin sequences are not yet known for baleen whales. We therefore used peptide mass fingerprinting to determine peptidic profiles in well documented baleen and evaluated the possibility of using this technique to differentiate species in baleen samples that are not adequately identified or are unidentified. We examined baleen from ten different species of whales and determined molecular markers for each species, including species-specific markers. In the case of the Bryde’s whales, differences between specimens suggest distinct species or sub-species, consistent with the complex phylogeny of the species. Finally, the methodology was applied to 29 fragments of baleen excavated from archaeological sites in Labrador, Canada (representing 1500 years of whale use by prehistoric people), demonstrating a dominance of bowhead whale (Balaena mysticetus) in the archaeological assemblage and the successful application of the peptide mass fingerprinting technique to identify the species of whale in unidentified and partially degraded samples.
机译：Baleen已被土著人民收割了数千年，并作为现代商业捕鲸的附加产品被捕鲸者收集。 Baleen是指Mysticeti鲸鱼的食物过滤系统；一个完整的baleen机架由数十个坚硬而柔韧的角蛋白材料制成的板块组成，这些板块以硬毛结尾。由于其特性，baleen是一种有价值的原材料，广泛用于从工具到服装的各种人工制品。 Baleen在今天并未得到广泛使用，但是，对这种生物分子组织的分析有可能有助于保护工作，遗传多样性的研究以及对过去和最近对Mysticeti鲸的开发和使用的更好理解。幸运的是，baleen大量存在于博物馆的自然历史收藏中。但是，通常很难或不可能对制成的或旧的蜡笔进行物种鉴定。在这里，我们提出一种新的工具，用于基于Baleen的主要结构成分α-角蛋白（构成头发和指甲的相同蛋白质）进行生物分子鉴定。除小须鲸外，尚不知道须鲸的α-角蛋白序列。因此，我们使用肽质量指纹图谱确定了文献充分证明的巴利恩中的肽谱，并评估了使用该技术区分巴利恩样品中未被充分鉴定或未鉴定的物种的可能性。我们检查了来自十个不同种类鲸鱼的Baleen，并确定了每个物种的分子标记，包括物种特异性标记。以布赖德鲸为例，标本之间的差异表明物种或亚种不同，这与该物种的复杂系统发育相一致。最后，该方法应用于从加拿大拉布拉多考古遗址出土的29片巴利安碎片（代表史前人类使用鲸鱼1500年），证明了弓头鲸（Balaena mysticetus）在考古组合中的优势地位和成功应用肽质量指纹技术可鉴定未鉴定和部分降解的样品中的鲸鱼种类。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号