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Foam separation of Rhodamine-G and Evans Blue using a simple separatory bottle system

机译：使用简单的分离瓶系统泡沫分离罗丹明-G和伊万思蓝

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摘要

A simple separatory glass bottle was used to improve separation effectiveness and cost efficiency while simultaneously creating a simpler system for separating biological compounds. Additionally, it was important to develop a scalable separation method so this would be applicable to both analytical and preparative separations. Compared to conventional foam separation methods, this method easily forms stable dry foam which ensures high purity of yielded fractions. A negatively charged surfactant, sodium dodecyl sulfate (SDS), was used as the ligand to carry a positively charged Rhodamine-G, leaving a negatively charged Evans Blue in the bottle. The performance of the separatory bottle was tested for separating Rhodamine-G from Evans Blue with sample sizes ranged from 1 to 12 mg in preparative separations and 1 to 20 μg in analytical separations under optimum conditions. These conditions including N2 gas pressure, spinning speed of contents with a magnetic stirrer, concentration of the ligand, volume of the solvent, and concentration of the sample, were all modified and optimized. Based on the calculations at their peak absorbances, Rhodamine-G and Evans Blue were efficiently separated in times ranging from 1 hour to 3 hours, depending on sample volume. Optimal conditions were found to be 60 psi N2 pressure and 2 mM SDS for the affinity ligand. This novel separation method will allow for rapid separation of biological compounds while simultaneously being scalable and cost effective.
机译：使用一个简单的玻璃瓶来提高分离效率和成本效率，同时创建一个用于分离生物化合物的简单系统。此外，开发可扩展的分离方法也很重要，因此这将适用于分析分离和制备分离。与传统的泡沫分离方法相比，该方法容易形成稳定的干泡沫，从而确保了所得馏分的高纯度。带负电荷的表面活性剂十二烷基硫酸钠（SDS）用作配体，以携带带正电荷的若丹明-G，并在瓶中留下带负电荷的伊文思蓝。在最佳条件下，测试了分离瓶用于从伊文思蓝中分离若丹明-G的性能，样品大小在制备性分离中为1至12 mg，在分析性分离中为1至20μg。这些条件包括氮气压力，用磁力搅拌器旋转内容物的旋转速度，配体的浓度，溶剂的体积以及样品的浓度，都经过了修改和优化。根据峰吸光度的计算，根据样品量，若丹明-G和伊文思蓝的有效分离时间为1小时至3小时。发现最佳条件是亲和配体的60 psi N2压力和2 mM SDS。这种新颖的分离方法将允许快速分离生物化合物，同时具有可扩展性和成本效益。

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