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首页> 美国卫生研究院文献>other >Transcriptome Analysis of Cell Wall and NAC Domain Transcription Factor Genes during Elaeis guineensis Fruit Ripening: Evidence for Widespread Conservation within Monocot and Eudicot Lineages
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Transcriptome Analysis of Cell Wall and NAC Domain Transcription Factor Genes during Elaeis guineensis Fruit Ripening: Evidence for Widespread Conservation within Monocot and Eudicot Lineages

机译：吉利鹅果实成熟过程中细胞壁和NAC域转录因子基因的转录组分析：单子叶植物和双子叶植物谱系中广泛保存的证据

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The oil palm (Elaeis guineensis), a monocotyledonous species in the family Arecaceae, has an extraordinarily oil rich fleshy mesocarp, and presents an original model to examine the ripening processes and regulation in this particular monocot fruit. Histochemical analysis and cell parameter measurements revealed cell wall and middle lamella expansion and degradation during ripening and in response to ethylene. Cell wall related transcript profiles suggest a transition from synthesis to degradation is under transcriptional control during ripening, in particular a switch from cellulose, hemicellulose, and pectin synthesis to hydrolysis and degradation. The data provide evidence for the transcriptional activation of expansin, polygalacturonase, mannosidase, beta-galactosidase, and xyloglucan endotransglucosylase/hydrolase proteins in the ripening oil palm mesocarp, suggesting widespread conservation of these activities during ripening for monocotyledonous and eudicotyledonous fruit types. Profiling of the most abundant oil palm polygalacturonase (EgPG4) and 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) transcripts during development and in response to ethylene demonstrated both are sensitive markers of ethylene production and inducible gene expression during mesocarp ripening, and provide evidence for a conserved regulatory module between ethylene and cell wall pectin degradation. A comprehensive analysis of NAC transcription factors confirmed at least 10 transcripts from diverse NAC domain clades are expressed in the mesocarp during ripening, four of which are induced by ethylene treatment, with the two most inducible (EgNAC6 and EgNAC7) phylogenetically similar to the tomato NAC-NOR master-ripening regulator. Overall, the results provide evidence that despite the phylogenetic distance of the oil palm within the family Arecaceae from the most extensively studied monocot banana fruit, it appears ripening of divergent monocot and eudicot fruit lineages are regulated by evolutionarily conserved molecular physiological processes.
机译：油棕（Elaeis guineensis）是槟榔科的单子叶植物，具有非常丰富的油性肉质中果皮，并提出了一个原始模型来检查这种特定单子叶植物果实的成熟过程和调控。组织化学分析和细胞参数测量表明，在成熟过程中以及对乙烯的反应中，细胞壁和中间薄片的膨胀和降解。细胞壁相关的转录物谱表明，从合成到降解的过渡在成熟过程中处于转录控制之下，特别是从纤维素，半纤维素和果胶合成到水解和降解的转变。数据提供了在成熟的油棕中果皮中扩展蛋白，聚半乳糖醛酸酶，甘露糖苷酶，β-半乳糖苷酶和木葡聚糖内切葡糖苷酶/水解酶蛋白的转录激活的证据，表明这些活性在单子叶和双子叶水果类型的成熟过程中得到了广泛的保存。在发育和响应乙烯过程中对最丰富的油棕多半乳糖醛酸酶（EgPG4）和1-氨基环丙烷-1-羧酸氧化酶（ACO）转录本进行谱分析表明，这两者都是中果皮成熟过程中乙烯生成和诱导型基因表达的敏感标志物，并提供乙烯和细胞壁果胶降解之间保守的调节模块的证据。对NAC转录因子的全面分析证实，成熟期间中果皮中至少有10个来自不同NAC域进化枝的转录物表达，其中有4个是通过乙烯处理诱导的，其中两种诱导型（EgNAC6和EgNAC7）的系统发育与番茄NAC相似。 -NOR主控调节器。总体而言，结果提供了证据，尽管槟榔科中的油棕与最广泛研究的单子叶香蕉果实之间的系统发育距离，似乎是由进化上保守的分子生理过程调节的。

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