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The analysis of viability for mammalian cells treated at different temperatures and its application in cell shipment

机译：不同温度处理的哺乳动物细胞的活力分析及其在细胞运输中的应用

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Mammalian cells are very important experimental materials and widely used in biological and medical research fields. It is often required that mammalian cells are transported from one laboratory to another to meet with various researches. Conventional methods for cell shipment are laborious and costive despite of maintaining high viability. In this study we aimed to develop a simple and low-cost method for cell shipment by investigating the viabilities of different cell lines treated at different temperatures. We show that the viability of mammalian cells incubated at 1°C or 5°C significantly reduced when compared with that at 16°C or 22°C. Colony formation assays revealed that preservation of mammalian cells at 1°C or 5°C led to a poorer recovery than that at 16°C or 22°C. The data from proliferation and apoptotic assays confirmed that M2 cells could continue to proliferate at 16°C or 22°C, but massive death was caused by apoptosis at 1°C or 5°C. The morphology of mammalian cells treated under hypothermia showed little difference from that of the untreated cells. Quantitative RT-PCR and alkaline phosphatase staining confirmed that hypothermic treatment did not change the identity of mouse embryonic stem cells. A case study showed that mammalian cells directly suspended in culture medium were able to be shipped for long distance and maintained a high level of viability and recovery. Our findings not only broaden the understanding to the effect of hypothermia on the viability of mammalian cells, but also provide an alternative approach for cell shipment.
机译：哺乳动物细胞是非常重要的实验材料，广泛用于生物学和医学研究领域。通常需要将哺乳动物细胞从一个实验室运送到另一个实验室，以满足各种研究的需要。尽管维持高生存力，用于细胞运输的常规方法费力且昂贵。在这项研究中，我们旨在通过研究在不同温度下处理的不同细胞系的生存能力，开发一种简单且低成本的细胞运输方法。我们显示，与在16°C或22°C相比，在1°C或5°C孵育的哺乳动物细胞的活力显着降低。集落形成试验表明，与16℃或22℃相比，在1℃或5℃保存哺乳动物细胞导致回收率较差。来自增殖和凋亡测定的数据证实，M2细胞可以在16℃或22℃继续增殖，但是大量死亡是由1℃或5℃的细胞凋亡引起的。在低温下处理的哺乳动物细胞的形态与未处理的细胞几乎没有差异。定量RT-PCR和碱性磷酸酶染色证实低温治疗不会改变小鼠胚胎干细胞的身份。案例研究表明，直接悬浮在培养基中的哺乳动物细胞能够长距离运输，并保持高水平的生存能力和恢复能力。我们的发现不仅拓宽了对体温过低对哺乳动物细胞活力影响的理解，而且为细胞运输提供了另一种方法。

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