• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>other >Fungal Root Microbiome from Healthy and Brittle Leaf Diseased Date Palm Trees (Phoenix dactylifera L.) Reveals a Hidden Untapped Arsenal of Antibacterial and Broad Spectrum Antifungal Secondary Metabolites
【2h】

Fungal Root Microbiome from Healthy and Brittle Leaf Diseased Date Palm Trees (Phoenix dactylifera L.) Reveals a Hidden Untapped Arsenal of Antibacterial and Broad Spectrum Antifungal Secondary Metabolites

机译：健康和脆性叶病枣棕榈树（Phoenix dactylifera L.）的真菌根微生物组揭示了一种抗菌和广谱抗真菌次生代谢产物的隐藏式未开发武器库。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study, we aimed to explore and compare the composition, metabolic diversity and antimicrobial potential of endophytic fungi colonizing internal tissues of healthy and brittle leaf diseased (BLD) date palm trees (Phoenix dactylifera L.) widely cultivated in arid zones of Tunisia. A total of 52 endophytic fungi were isolated from healthy and BLD roots of date palm trees, identified based on internal transcribed spacer-rDNA sequence analysis and shown to represent 13 species belonging to five genera. About 36.8% of isolates were shared between healthy and diseased root fungal microbiomes, whereas 18.4 and 44.7% of isolates were specific to healthy and BLD root fungal microbiomes, respectively. All isolates were able to produce at least two of the screened enzymes including amylase, cellulase, chitinase, pectinase, protease, laccase and lipase. A preliminary screening of the isolates using disk diffusion method for antibacterial activity against four Gram-positive and three Gram-negative bacteria and antifungal activities against three phytopathogenic fungi indicated that healthy and BLD root fungal microbiomes displayed interesting bioactivities against examined bacteria and broad spectrum bioactivity against fungal pathogens. Some of these endophytic fungi (17 isolates) were fermented and their extracts were evaluated for antimicrobial potential against bacterial and fungal isolates. Results revealed that fungal extracts exhibited antibacterial activities and were responsible for approximately half of antifungal activities against living fungi. These results suggest a strong link between fungal bioactivities and their secondary metabolite arsenal. EtOAc extracts of Geotrichum candidum and Thielaviopsis punctulata originating from BLD microbiome gave best results against Micrococcus luteus and Bacillus subtilis with minimum inhibitory concentration (MIC, 0.78 mg/mL) and minimum bactericidal concentration (6.25 mg/mL). G. candidum gave the best result against Rhizoctonia solani with MIC 0.78 mg/mL and minimum fungicidal concentration (MFC, 6.25 mg/mL). In conclusion, using plant microbiomes subjected to biotic stresses offers new endophytes with different bioactivities than those of healthy plants. Therefore, date palm endophytic fungi represent a hidden untapped arsenal of antibacterial and broad spectrum antifungal secondary metabolites and could be considered promising source of bioactive compounds with industrial and pharmaceutical applications.
机译：在这项研究中，我们旨在探索和比较在突尼斯干旱地区广泛种植的健康和脆性叶片病（BLD）枣树（Phoenix dactylifera L.）的内部组织中内生真菌的成分，代谢多样性和抗菌潜力。根据内部转录间隔物-rDNA序列分析，从枣树的健康和BLD根中分离出总共52种内生真菌，并显示它们代表了5个属的13种。健康和患病的根真菌微生物群共有大约36.8％的分离株，而健康和BLD根真菌微生物群则分别有18.4和44.7％的分离株。所有分离物均能够产生至少两种筛选的酶，包括淀粉酶，纤维素酶，几丁质酶，果胶酶，蛋白酶，漆酶和脂肪酶。使用圆盘扩散法对菌株进行的初步筛选对四种革兰氏阳性细菌和三种革兰氏阴性细菌具有抗菌活性，并对三种植物病原真菌具有抗真菌活性，这表明健康和BLD根真菌微生物群对受检细菌表现出有趣的生物活性，而对细菌的广谱生物活性具有显着性真菌病原体。发酵其中的一些内生真菌（17种分离株），评估其提取物对细菌和真菌分离株的抗菌潜力。结果表明，真菌提取物表现出抗菌活性，并且约占抗真菌活性的一半。这些结果表明，真菌的生物活性与其次生代谢产物武库之间有很强的联系。源自BLD微生物组的念珠菌和点状拟南芥的EtOAc提取物以最小的抑菌浓度（MIC，0.78 mg / mL）和最小的杀菌浓度（6.25 mg / mL）获得了针对黄曲霉和枯草芽孢杆菌的最佳结果。 G. candidum以MIC 0.78 mg / mL和最小杀真菌浓度（MFC，6.25 mg / mL）提供了对抗茄枯萎病的最佳结果。总而言之，使用受到生物胁迫的植物微生物群落可提供具有与健康植物不同的生物活性的新内生菌。因此，椰枣内生真菌代表了抗菌和广谱抗真菌次生代谢产物的一个未开发的潜在武器库，可以被认为在工业和制药应用中是有希望的生物活性化合物来源。

著录项

相似文献

  • 外文文献
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号