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Student Award for Outstanding Research Winner in the Ph.D. Category for the 2017 Society for Biomaterials Annual Meeting and Exposition April 5–8 2017 Minneapolis Minnesota

机译：博士杰出研究奖学生奖2017年生物材料学会年会和博览会的类别2017年4月5日至8日明尼苏达州明尼阿波利斯

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Molecularly imprinted polymers (MIPs) with selective affinity for protein biomarkers could find extensive utility as environmentally robust, cost-efficient biomaterials for diagnostic and therapeutic applications. In order to develop recognitive, synthetic biomaterials for prohibitively expensive protein biomarkers, we have developed a molecular imprinting technique that utilizes structurally similar, analogue proteins. Hydrogel microparticles synthesized by molecular imprinting with trypsin, lysozyme, and cytochrome c possessed an increased affinity for alternate high isoelectric point biomarkers both in isolation and plasma-mimicking adsorption conditions. Imprinted and non-imprinted P(MAA-co-AAm-co-DEAEMA) microgels containing PMAO-PEGMA functionalized polycaprolactone nanoparticles were net-anionic, polydisperse, and irregularly shaped. MIPs and control non-imprinted polymers (NIPs) exhibited regions of Freundlich and BET isotherm adsorption behavior in a range of non-competitive protein solutions, where MIPs exhibited enhanced adsorption capacity in the Freundlich isotherm regions. In a competitive condition, imprinting with analogue templates (trypsin, lysozyme) increased the adsorption capacity of microgels for cytochrome c by 162% and 219%, respectively, as compared to a 122% increase provided by traditional bulk imprinting with cytochrome c. Our results suggest that molecular imprinting with analogue protein templates is a viable synthetic strategy for enhancing hydrogel-biomarker affinity and promoting specific protein adsorption behavior in biological fluids.
机译：对蛋白质生物标志物具有选择性亲和力的分子印迹聚合物（MIP）可以广泛用作诊断和治疗应用的环保，经济高效的生物材料。为了开发用于价格昂贵的蛋白质生物标记物的识别性合成生物材料，我们开发了一种分子印迹技术，该技术利用结构相似的类似蛋白质。通过胰蛋白酶，溶菌酶和细胞色素c的分子印迹合成的水凝胶微粒，在分离和模拟血浆的吸附条件下，对其他高等电点生物标记物的亲和力均增加。包含PMAO-PEGMA功能化聚己内酯纳米颗粒的印迹和非印迹P（MAA-co-AAm-co-DEAEMA）微凝胶为净阴离子型，多分散型和不规则形状。在一系列非竞争性蛋白质溶液中，MIP和对照非印迹聚合物（NIP）表现出Freundlich和BET等温线吸附行为，其中MIP在Freundlich等温线区域表现出增强的吸附能力。在竞争条件下，与类似物模板（胰蛋白酶，溶菌酶）的印迹使微凝胶对细胞色素c的吸附能力分别增加了162％和219％，而传统的批量与细胞色素c的印迹增加了122％。我们的结果表明，具有类似蛋白质模板的分子印迹是增强水凝胶-生物标志物亲和力和促进生物流体中特定蛋白质吸附行为的可行合成策略。

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