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Instrument design and protocol for the study of light controlled processes in aquatic organisms and its application to examine the effect of infrared light on zebrafish

机译：用于研究水生生物的光控制过程的仪器设计和协议及其在检查红外光对斑马鱼的影响中的应用

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摘要

The acquisition of reliable data strongly depends on experimental design. When studying the effects of light on processes such as behaviour and physiology it is crucial to maintain all environmental conditions constant apart from the one under study. Furthermore, the precise values of the environmental factors applied during the experiment should be known. Although seemingly obvious, these conditions are often not met when the effects of light are being studied. Here, we document and discuss the wavelengths and light intensities of natural and artificial light sources. We present standardised experimental protocols together with building plans of a custom made instrument designed to accurately control light and temperature for experiments using fresh water or marine species. Infrared light is commonly used for recording behaviour and in electrophysiological experiments although the properties of fish photoreceptors potentially allow detection into the far red. As an example of our experimental procedure we have applied our protocol and instrument to specifically test the impact of infrared light (840 nm) on the zebrafish circadian clock, which controls many aspects of behaviour, physiology and metabolism. We demonstrate that infrared light does not influence the zebrafish circadian clock. Our results help to provide a solid framework for the future study of light dependent processes in aquatic organisms.
机译：可靠数据的获取很大程度上取决于实验设计。在研究光对行为和生理等过程的影响时，至关重要的是保持所有环境条件始终处于恒定状态。此外，应该知道实验期间应用的环境因素的精确值。尽管看似显而易见，但在研究光的影响时通常无法满足这些条件。在这里，我们记录和讨论自然和人造光源的波长和光强度。我们提供标准化的实验方案以及定制仪器的构建计划，该仪器旨在精确控制淡水或海洋物种实验的光和温度。红外光通常用于记录行为和进行电生理实验，尽管鱼感光体的特性可能允许将其检测为远红色。作为我们实验程序的一个例子，我们已经应用了方案和仪器专门测试了红外光（840 nm）对斑马鱼生物钟的影响，该生物钟控制着行为，生理学和新陈代谢的许多方面。我们证明红外光不会影响斑马鱼的生物钟。我们的结果有助于为未来水生生物中光依赖性过程的研究提供一个坚实的框架。

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