• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>other >Surface Acoustic Waves Induced Micropatterning of Cells in Gelatin Methacryloyl (GelMA) Hydrogels
【2h】

Surface Acoustic Waves Induced Micropatterning of Cells in Gelatin Methacryloyl (GelMA) Hydrogels

机译：表面声波诱导明胶甲基丙烯酰基（GelMA）水凝胶中的细胞微图案。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Acoustic force patterning is an emerging technology that provides a platform to control the spatial location of cells in a rapid, accurate, yet contactless manner. However, very few studies have been reported on the usage of acoustic force patterning for the rapid arrangement of biological objects, such as cells, in a three-dimensional (3D) environment. In this study, we report on a bio-acoustic force patterning technique, which uses surface acoustic waves (SAWs) for the rapid arrangement of cells within an extracellular matrix (ECM)-based hydrogel such as gelatin methacryloyl (GelMA). A proof-of-principle was achieved through both simulations and experiments based on the in-house fabricated piezoelectric SAW transducers, which enabled us to explore the effects of various parameters on the performance of the built construct. The SAWs were applied in a fashion that generated standing SAWs (SSAWs) on the substrate, the energy of which subsequently was transferred into the gel, creating a rapid, yet contactless alignment of the cells (< 10 seconds, based on the experimental conditions). Following UV radiation induced photo-crosslinking of the cell encapsulated GelMA pre-polymer solution, the patterned cardiac cells readily spread after alignment in the GelMA hydrogel and demonstrated beating activity in 5-7 days. The described acoustic force assembly method can be utilized not only to control the spatial distribution of the cells inside a 3D construct, but can also preserve the viability and functionality of the patterned cells (e.g. beating rates of cardiac cells). This platform can be potentially employed in a diverse range of applications, whether it is for tissue engineering, in vitro cell studies, or creating 3D biomimetic tissue structures.
机译：声力图案化是一种新兴技术，它提供了一个平台来以快速，准确但无接触的方式控制细胞的空间位置。但是，关于声力图案在三维（3D）环境中快速布置生物物体（例如细胞）的用途的报道很少。在这项研究中，我们报告了一种生物声力图案化技术，该技术使用表面声波（SAW）在基于细胞外基质（ECM）的水凝胶（例如明胶甲基丙烯酰基（GelMA））中快速排列细胞。通过基于内部制造的压电声表面波换能器的仿真和实验均获得了原理证明，这使我们能够探索各种参数对构建结构性能的影响。以表面上产生站立SAW（SSAW）的方式施加SAW，随后将其能量转移到凝胶中，从而使细胞快速但无接触地对准（根据实验条件，<10秒） 。在紫外线辐射诱导的细胞封装的GelMA预聚物溶液发生光交联之后，图案化的心脏细胞在GelMA水凝胶中对齐后易于扩散，并在5-7天内表现出跳动活性。所描述的声力组装方法不仅可以用于控制3D构造内部细胞的空间分布，而且还可以保留图案化细胞的生存力和功能性（例如，心脏细胞的跳动率）。无论是用于组织工程，体外细胞研究，还是创建3D仿生组织结构，该平台都可以广泛应用于各种应用中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号