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首页> 美国卫生研究院文献>other >Reconstruction of the Metabolic Potential of Acidophilic Sideroxydans Strains from the Metagenome of an Microaerophilic Enrichment Culture of Acidophilic Iron-Oxidizing Bacteria from a Pilot Plant for the Treatment of Acid Mine Drainage Reveals Metabolic Versatility and Adaptation to Life at Low pH
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Reconstruction of the Metabolic Potential of Acidophilic Sideroxydans Strains from the Metagenome of an Microaerophilic Enrichment Culture of Acidophilic Iron-Oxidizing Bacteria from a Pilot Plant for the Treatment of Acid Mine Drainage Reveals Metabolic Versatility and Adaptation to Life at Low pH

机译：从试验性工厂中的酸性嗜酸性氧化铁细菌的微需氧富集培养物的基因组重建嗜酸性五味子系的代谢潜能用于处理酸性矿山排水揭示了代谢性和适应低pH条件下的生活。

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Bacterial community analyses of samples from a pilot plant for the treatment of acid mine drainage (AMD) from the lignite-mining district in Lusatia (East Germany) had previously demonstrated the dominance of two groups of acidophilic iron oxidizers: the novel candidate genus “Ferrovum” and a group comprising Gallionella-like strains. Since pure culture had proven difficult, previous studies have used genome analyses of co-cultures consisting of “Ferrovum” and a strain of the heterotrophic acidophile Acidiphilium in order to obtain insight into the life style of these novel bacteria. Here we report on attempts to undertake a similar study on Gallionella-like acidophiles from AMD. Isolates belonging to the family Gallionellaceae are still restricted to the microaerophilic and neutrophilic iron oxidizers Sideroxydans and Gallionella. Availability of genomic or metagenomic sequence data of acidophilic strains of these genera should, therefore, be relevant for defining adaptive strategies in pH homeostasis. This is particularly the case since complete genome sequences of the neutrophilic strains G. capsiferriformans ES-2 and S. lithotrophicus ES-1 permit the direct comparison of the metabolic capacity of neutrophilic and acidophilic members of the same genus and, thus, the detection of biochemical features that are specific to acidophilic strains to support life under acidic conditions. Isolation attempts undertaken in this study resulted in the microaerophilic enrichment culture ADE-12-1 which, based on 16S rRNA gene sequence analysis, consisted of at least three to four distinct Gallionellaceae strains that appear to be closely related to the neutrophilic iron oxidizer S. lithotrophicus ES-1. Key hypotheses inferred from the metabolic reconstruction of the metagenomic sequence data of these acidophilic Sideroxydans strains include the putative role of urea hydrolysis, formate oxidation and cyanophycin decarboxylation in pH homeostasis.
机译：细菌群落对来自卢萨蒂亚（东德）褐煤开采区的酸性矿山排水中试工厂（AMD）样品的细菌分析先前证明了两组嗜酸性铁氧化剂的优势：新的候选属“铁矾”和一组包含Gallionella样菌株的菌株。由于事实证明纯培养是困难的，以前的研究已经使用了由“费洛姆氏菌”和嗜酸嗜酸性嗜酸菌属菌株组成的共培养物的基因组分析，以便深入了解这些新型细菌的生活方式。在这里，我们报道了尝试对AMD的Gallionella类嗜酸菌进行类似研究的尝试。隶属于镓菌科的分离物仍限于微需氧和嗜中性的铁氧化剂Sideroxydans和Gallionella。因此，这些属的嗜酸菌株的基因组或宏基因组序列数据的可用性应与定义pH稳态的适应策略有关。特别是这种情况，因为嗜中性菌株Capsiferriformans ES-2和S. lithotrophicus ES-1的完整基因组序列可以直接比较同一属的嗜中性和嗜酸性成员的代谢能力，从而检测嗜酸菌株特有的生化特征，可在酸性条件下维持生命。在这项研究中进行的分离尝试导致了微需氧富集培养物ADE-12-1，该培养物基于16S rRNA基因序列分析，由至少三至四个不同的胆囊菌科菌株组成，这些菌株似乎与嗜中性铁氧化剂S密切相关。岩溶菌ES-1。从这些嗜酸性Sideroxydans菌株宏基因组序列数据的代谢重建中得出的关键假设包括：尿素水解，甲酸盐氧化和蓝霉素脱羧在pH稳态中的假定作用。

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