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首页> 美国卫生研究院文献>other >Soymilk residue (okara) as a natural immobilization carrier for Lactobacillus plantarum cells enhances soymilk fermentation glucosidic isoflavone bioconversion and cell survival under simulated gastric and intestinal conditions
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Soymilk residue (okara) as a natural immobilization carrier for Lactobacillus plantarum cells enhances soymilk fermentation glucosidic isoflavone bioconversion and cell survival under simulated gastric and intestinal conditions

机译：豆浆残留物（豆渣）作为植物乳杆菌细胞的天然固定载体可增强豆浆发酵糖异黄酮的生物转化以及在模拟的胃和肠条件下的细胞存活率

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Cell immobilization is an alternative to microencapsulation for the maintenance of cells in a liquid medium. However, artificial immobilization carriers are expensive and pose a high safety risk. Okara, a food-grade byproduct from soymilk production, is rich in prebiotics. Lactobacilli could provide health enhancing effects to the host. This study aimed to evaluate the potential of okara as a natural immobilizer for L. plantarum 70810 cells. The study also aimed to evaluate the effects of okara-immobilized L. plantarum 70810 cells (IL) on soymilk fermentation, glucosidic isoflavone bioconversion, and cell resistance to simulated gastric and intestinal stresses. Scanning electron microscopy (SEM) was used to show cells adherence to the surface of okara. Lactic acid, acetic acid and isoflavone analyses in unfermented and fermented soymilk were performed by HPLC with UV detection. Viability and growth kinetics of immobilized and free L. plantarum 70810 cells (FL) were followed during soymilk fermentation. Moreover, changes in pH, titrable acidity and viscosity were measured by conventional methods. For in vitro testing of simulated gastrointestinal resistance, fermented soymilk was inoculated with FL or IL and an aliquot incubated into acidic MRS broth which was conveniently prepared to simulate gastric, pancreatic juices and bile salts. Survival to simulated gastric and intestinal stresses was evaluated by plate count of colony forming units on MRS agar. SEM revealed that the lactobacilli cells attached and bound to the surface of okara. Compared with FL, IL exhibited a significantly higher specific growth rate, shorter lag phase of growth, higher productions of lactic and acetic acids, a faster decrease in pH and increase in titrable acidity, and a higher soymilk viscosity. Similarly, IL in soymilk showed higher productions of daizein and genistein compared with the control. Compared with FL, IL showed reinforced resistance to simulatedgastric and intestinal stresses in vitro that included low pH, low pH plus pepsin, pancreatin, and bile salt. Our results indicate that okara is a new potential immobilization carrier to enhance the growth and glucosidic isoflavone bioconversion activities of L. plantarum in soymilk and improve cell survivability following simulated gastric and intestinal conditions.
机译：细胞固定是微囊化的替代方法，用于在液体培养基中维持细胞。但是，人工固定载体价格昂贵并且存在很高的安全风险。豆奶生产的食品级副产品豆渣富含益生元。乳酸杆菌可以为宿主提供健康促进作用。这项研究旨在评估豆渣作为植物乳杆菌70810细胞天然固定剂的潜力。该研究还旨在评估豆渣固定的植物乳杆菌70810细胞（IL）对豆浆发酵，糖苷异黄酮生物转化以及细胞对模拟的胃和肠压力的抵抗力。扫描电子显微镜（SEM）用于显示细胞粘附于豆渣表面。通过HPLC和UV检测对未发酵和发酵豆浆中的乳酸，乙酸和异黄酮进行分析。在豆浆发酵过程中，跟踪固定的和游离的植物乳杆菌70810细胞（FL）的活力和生长动力学。此外，通过常规方法测量pH，可滴定酸度和粘度的变化。为了在体外测试模拟的胃肠道抵抗力，将发酵的豆浆接种FL或IL，然后将等分试样孵育到酸性MRS肉汤中，该肉汤可方便地制备以模拟胃，胰液和胆汁盐。通过在MRS琼脂上菌落形成单位的平板计数来评估模拟胃和肠应激的存活率。扫描电镜显示，乳酸杆菌细胞附着并结合在豆渣表面。与FL相比，IL表现出明显更高的比生长速率，较短的生长滞后阶段，较高的乳酸和乙酸产量，更快的pH降低速度和可滴定的酸度增加以及较高的豆浆粘度。同样，豆浆中的IL与对照相比显示出更高的黄豆素和染料木黄素产量。与FL相比，IL在体外对模拟的胃和肠应激表现出增强的抵抗力，包括低pH，低pH加上胃蛋白酶，胰酶和胆汁盐。我们的结果表明，豆渣是一种新的潜在固定化载体，可提高豆乳中植物乳杆菌的生长和糖苷异黄酮的生物转化活性，并在模拟的胃和肠条件下提高细胞存活率。

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