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THE VASELINE TUBE AND SYRINGE METHOD OF MICRO GAS ANALYSIS OF BACTERIAL CULTURES

机译:细菌培养物微气体分析的瓶管和进样方法

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摘要

There has been described the use of the vaseline tube and the tuberculin syringe for the study of gas production by bacteria. A comparison is made of some of the results obtained by the use of the method here described, the Smith fermentation tube, and the tube of Eldredge and Rogers. The reports of CO2 production by certain streptococci by Ayers, Rupp, and Mudge and by Bacterium typhosus by Nichols have been confirmed by the author's method. The data presented serve to illustrate the accuracy and technical possibilities of the method. In addition to economy of glassware, medium, and labor, the vaseline tube and syringe method of micro gas analysis possesses the following advantages. (1) Gas produced above either liquid or solid media may be measured and analyzed. (2) The gas produced may be measured in terms of a definite and constant quantity of medium used. (3) The vaseline tube provides a closed system from which gases do not escape into the air. (4) Separate determinations of the CO2 produced in and above fluid media may be made. (5) Determinations may be made from very small samples of material. (6) Numerous gas analyses of the same culture may be made at various times during the growth of the culture without contaminating or destroying it. (7) Gas production may be observed under both anaerobic and controlled aerobic conditions.
机译:已经描述了凡士林管和结核菌素注射器用于研究细菌产生的气体的用途。比较了使用此处描述的方法,Smith发酵管以及Eldredge和Rogers试管获得的一些结果。作者的方法证实了艾尔斯(Ayers),鲁普(Rupp)和马奇(Mudge)某些链球菌产生的二氧化碳报告,尼科尔斯(Nichols)产生的伤寒细菌报告了二氧化碳。所提供的数据用于说明该方法的准确性和技术可能性。除玻璃器皿的经济,培养基和劳力外,微气体分析的凡士林管和注射器方法还具有以下优点。 (1)可以测量和分析在液体或固体介质上方产生的气体。 (2)产生的气体可以根据确定的和恒定的使用介质量进行测量。 (3)凡士林管提供了一个密闭系统,气体不会从该系统逸出到空气中。 (4)可以分别确定在流体介质中和上方产生的CO2。 (5)可以从很小的材料样本中进行测定。 (6)在培养物生长期间,可以在不同时间对同一培养物进行大量气体分析,而不会污染或破坏该培养物。 (7)在厌氧和受控有氧条件下均可观察到气体产生。

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