• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>other >Magnetic Microbead Affinity Selection Screening (MagMass) of Botanical Extracts for Inhibitors of 15-Lipoxygenase
【2h】

Magnetic Microbead Affinity Selection Screening (MagMass) of Botanical Extracts for Inhibitors of 15-Lipoxygenase

机译：植物提取物对15-Lipoxygenase抑制剂的磁性微珠亲和力选择筛选（MagMass）

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To expedite the identification of active natural products in complex mixtures such as botanical extracts, a Magnetic Microbead Affinity Selection Screening (MagMASS) procedure was developed. This technique utilizes target proteins immobilized on magnetic beads for rapid bioaffinity isolation of ligands from complex mixtures. A MagMASS method was developed and validated for 15-lipoxygenase. As a proof of concept, several North American prairie plants used medicinally by Native Americans were extracted with MeOH and screened. A hit from an extract of Proserpinaca palustris, also known as mermaid weed, was flagged for further characterization using high-resolution tandem mass spectrometry, dereplication, and identification using XCMS online. Through the application of high-resolution product ion tandem mass spectrometry, comparison with natural product databases and confirmation using standards, the hit was identified as quercitrin, which is a known inhibitor of 15-lipoxygenase. The overall workflow of MagMASS is faster and more amendable to automation than alternative methods designed for screening botanical extracts or complex mixtures of combinatorial libraries.
机译：为了加快鉴定复杂混合物（例如植物提取物）中的活性天然产物，开发了磁微珠亲和力选择筛选（MagMASS）程序。该技术利用固定在磁珠上的靶蛋白从复杂混合物中快速生物亲和分离配体。开发了MagMASS方法并验证了15-脂氧合酶的有效性。作为概念的证明，用MeOH提取了几种北美印第安人药用的草原植物，并进行了筛选。来自Proserpinaca palustris提取物（也称为美人鱼杂草）的命中被标记为使用高分辨率串联质谱，重复复制和在线XCMS鉴定进行进一步表征。通过高分辨率产物离子串联质谱的应用，与天然产物数据库的比较以及使用标准品的确认，该命中被鉴定为槲皮苷，其是一种已知的15-脂氧合酶抑制剂。与为筛选植物提取物或组合库的复杂混合物而设计的替代方法相比，MagMASS的整体工作流程更快，更易于自动化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号