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首页> 美国卫生研究院文献>other >RAP2.4a Is Transported through the Phloem to Regulate Cold and Heat Tolerance in Papaya Tree (Carica papaya cv. Maradol): Implications for Protection Against Abiotic Stress
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RAP2.4a Is Transported through the Phloem to Regulate Cold and Heat Tolerance in Papaya Tree (Carica papaya cv. Maradol): Implications for Protection Against Abiotic Stress

机译：RAP2.4a通过韧皮部运输来调节木瓜树（Carica papaya cv。Maradol）的耐寒性和耐热性：预防非生物胁迫的意义

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Plants respond to stress through metabolic and morphological changes that increase their ability to survive and grow. To this end, several transcription factor families are responsible for transmitting the signals that are required for these changes. Here, we studied the transcription factor superfamily AP2/ERF, particularly, RAP2.4 from Carica papaya cv. Maradol. We isolated four genes (CpRap2.4a, CpRAap2.4b, CpRap2.1 and CpRap2.10), and an in silico analysis showed that the four genes encode proteins that contain a conserved APETALA2 (AP2) domain located within group I and II transcription factors of the AP2/ERF superfamily. Semiquantitative PCR experiments indicated that each CpRap2 gene is differentially expressed under stress conditions, such as extreme temperatures. Moreover, genetic transformants of tobacco plants overexpressing CpRap2.4a and CpRap2.4b genes show a high level of tolerance to cold and heat stress compared to non-transformed plants. Confocal microscopy analysis of tobacco transgenic plants showed that CpRAP2.4a and CpRAP2.4b proteins were mainly localized to the nuclei of cells from the leaves and roots and also in the sieve elements. Moreover, the movement of CpRap2.4a RNA in tobacco grafting was analyzed. Our results indicate that CpRap2.4a and CpRap2.4b RNA in the papaya tree have a functional role in the response to stress conditions such as exposure to extreme temperatures via direct translation outside the parental RNA cell.
机译：植物通过新陈代谢和形态变化来应对压力，从而增加其生存和生长的能力。为此，几个转录因子家族负责传输这些变化所需的信号。在这里，我们研究了转录因子超家族AP2 / ERF，尤其是番木瓜的RAP2.4。马拉多我们分离了四个基因（CpRap2.4a，CpRAap2.4b，CpRap2.1和CpRap2.10），并且计算机分析表明这四个基因编码的蛋白质均包含位于I和II组转录中的保守APETALA2（AP2）域AP2 / ERF超家族的因素。半定量PCR实验表明，每个CpRap2基因在胁迫条件下（例如极端温度）均差异表达。而且，与未转化的植物相比，过量表达CpRap2.4a和CpRap2.4b基因的烟草植物的遗传转化体显示出对冷和热胁迫的高度耐受性。烟草转基因植物的共聚焦显微镜分析表明，CpRAP2.4a和CpRAP2.4b蛋白主要位于叶和根的细胞核中，也位于筛子中。此外，分析了CpRap2.4a RNA在烟草嫁接中的运动。我们的结果表明，木瓜树中的CpRap2。 4a 和 CpRap2 。 4b RNA在对胁迫条件（如暴露）的响应中具有功能性作用通过亲本RNA细胞外的直接翻译达到极端温度。

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