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Comparative Transcriptome Analysis of Latex Reveals Molecular Mechanisms Underlying Increased Rubber Yield in Hevea brasiliensis Self-Rooting Juvenile Clones

机译：乳胶的对比转录组分析揭示了橡胶树巴西自生幼株橡胶产量增加的分子机理

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Rubber tree (Hevea brasiliensis) self-rooting juvenile clones (JCs) are promising planting materials for rubber production. In a comparative trial between self-rooting JCs and donor clones (DCs), self-rooting JCs exhibited better performance in rubber yield. To study the molecular mechanism associated with higher rubber yield in self-rooting JCs, we sequenced and comparatively analyzed the latex of rubber tree self-rooting JCs and DCs at the transcriptome level. Total raw reads of 34,632,012 and 35,913,020 bp were obtained from the library of self-rooting JCs and DCs, respectively, by using Illumina HiSeq 2000 sequencing technology. De novo assemblies yielded 54689 unigenes from the library of self-rooting JCs and DCs. Among 54689 genes, 1716 genes were identified as differentially expressed between self-rooting JCs and DCs via comparative transcript profiling. Functional analysis showed that the genes related to the mass of categories were differentially enriched between the two clones. Several genes involved in carbohydrate metabolism, hormone metabolism and reactive oxygen species scavenging were up-regulated in self-rooting JCs, suggesting that the self-rooting JCs provide sufficient molecular basis for the increased rubber yielding, especially in the aspects of improved latex metabolisms and latex flow. Some genes encoding epigenetic modification enzymes were also differentially expressed between self-rooting JCs and DCs. Epigenetic modifications may lead to gene differential expression between self-rooting JCs and DCs. These data will provide new cues to understand the molecular mechanism underlying the improved rubber yield of H. brasiliensis self-rooting clones.
机译：橡胶树（巴西橡胶树）自生幼体（JCs）是用于橡胶生产的有前途的种植材料。在自生根JC和供体克隆（DC）之间的比较试验中，自生JC在橡胶产量方面表现出更好的性能。为了研究自生根JC中较高橡胶产量的分子机理，我们在转录组水平上对橡胶树自生JC和DC的胶乳进行了测序和比较分析。通过使用Illumina HiSeq 2000测序技术，分别从自生根JC和DC的库中获得了34,632,012和35,913,020 bp的总原始读数。从头组装从自生根的JC和DC的库中产生了54689个单基因。在54689个基因中，通过比较转录谱分析鉴定出1716个基因在自发JC和DC之间差异表达。功能分析表明，与这两个类别的质量相关的基因在两个克隆之间差异性富集。自生根JCs中涉及碳水化合物代谢，激素代谢和清除活性氧的几个基因被上调，表明自生根JCs为增加橡胶产量提供了足够的分子基础，尤其是在改善胶乳代谢和乳胶流。在自生JC和DC之间，一些编码表观遗传修饰酶的基因也有差异表达。表观遗传修饰可能导致自生根的JC和DC之间的基因差异表达。这些数据将提供新的线索，以了解提高巴西布鲁氏菌自生克隆的橡胶产量的分子机制。

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