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Training in metabolomics research. II. Processing and statistical analysis of metabolomics data metabolite identification pathway analysis applications of metabolomics and its future

机译:代谢组学研究培训。二。代谢组学数据的处理和统计分析代谢物鉴定途径分析代谢组学的应用及其未来

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摘要

Metabolomics, a systems biology discipline representing analysis of known and unknown pathways of metabolism, has grown tremendously over the past 20 years. Because of its comprehensive nature, metabolomics requires careful consideration of the question(s) being asked, the scale needed to answer the question(s), collection and storage of the sample specimens, methods for extraction of the metabolites from biological matrices, the analytical method(s) to be employed and the quality control of the analyses, how collected data are correlated, the statistical methods to determine metabolites undergoing significant change, putative identification of metabolites, and the use of stable isotopes to aid in verifying metabolite identity and establishing pathway connections and fluxes. This second part of a comprehensive description of the methods of metabolomics focuses on data analysis, emerging methods in metabolomics and the future of this discipline.
机译:代谢组学是一门系统生物学学科,代表对已知和未知代谢途径的分析,在过去20年中发展迅速。由于代谢组学的综合性质,需要仔细考虑所要提出的问题,回答问题所需的规模,样品标本的收集和储存,从生物基质中提取代谢物的方法,分析方法。所采用的方法和分析的质量控制,如何关联收集的数据,确定发生重大变化的代谢物的统计方法,假定的代谢物鉴定以及使用稳定的同位素来帮助验证代谢物的身份和建立通路连接和通量。代谢组学方法的全面描述的第二部分侧重于数据分析,代谢组学中的新兴方法以及该学科的未来。

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