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Biomimetic Hydrogel with Tunable Mechanical Properties for Vitreous Substitutes

机译：具有可调机械性能的玻璃仿生仿生水凝胶

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The vitreous humor of the eye is a biological hydrogel principally composed of collagen fibers interspersed with hyaluronic acid. Certain pathological conditions necessitate its removal and replacement. Current substitutes, like silicone oils and perfluorocarbons, are not biomimetic and have known complications. In this study, we have developed an in situ forming two-component biomimetic hydrogel with tunable mechanical and osmotic properties. The components are gellan, an analogue of collagen, and poly(methacrylamide-co-methacrylate), an analogue of hyaluronic acid; both endowed with thiol side groups. We used response surface methodology to consider seventeen possible hydrogels to determine how each component affects the optical, mechanical, sol-gel transition temperature and swelling properties. The optical and physical properties of the hydrogels were similar to vitreous. The shear storage moduli ranged from 3 to 358 Pa at 1Hz and sol-gel transition temperatures from 35.5 to 43 °C. The hydrogel had the ability to remain swollen without degradation for four weeks in vitro. Three hydrogels were tested for biocompatibility on primary porcine retinal pigment epithelial cells, human retinal pigment epithelial cells, and fibroblast (3T3/NIH) cells, by electric cell-substrate impedance sensing system. The two-component hydrogels allowed for the tuning and optimizing of mechanical, swelling, and transition temperature to obtain three biocompatible hydrogels with properties similar to vitreous. Future studies include testing of the optimized hydrogels in animal models for use as a long-term substitute, whose preliminary results are mentioned.
机译：眼睛的玻璃体液是一种生物水凝胶，主要由散布有透明质酸的胶原纤维组成。某些病理状况必须将其切除和更换。当前的替代品，例如硅油和全氟化碳，不是仿生的，并且具有已知的并发症。在这项研究中，我们开发了一种具有可调的机械和渗透特性的原位形成两组分仿生水凝胶。成分是结冷胶（一种胶原蛋白的类似物）和聚（甲基丙烯酰胺-甲基丙烯酸甲酯），一种透明质酸的类似物。两者都具有硫醇侧基。我们使用响应表面方法研究了十七种可能的水凝胶，以确定每种组分如何影响光学，机械，溶胶-凝胶转变温度和溶胀性能。水凝胶的光学和物理性质类似于玻璃质。 1Hz时的剪切储能模量为3至358 Pa，溶胶-凝胶转变温度为35.5至43°C。该水凝胶具有在体外保持肿胀而不降解四周的能力。通过电-细胞-基底阻抗传感系统，测试了三种水凝胶在原代猪视网膜色素上皮细胞，人视网膜色素上皮细胞和成纤维细胞（3T3 / NIH）细胞上的生物相容性。两组分水凝胶可调节和优化机械，溶胀和转变温度，以获得三种具有与玻璃体相似的生物相容性水凝胶。未来的研究包括在动物模型中测试优化的水凝胶，以用作长期替代品，并提及其初步结果。

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