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Application of response surface methodology and spectroscopic approach for investigating of curcumin nanoencapsulation using natural biopolymers and nonionic surfactant

机译:响应表面方法和光谱方法在研究天然生物聚合物和非离子表面活性剂姜黄素纳米胶囊的应用

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摘要

This study aimed at encapsulation of poor water-soluble curcumin so that it could be utilized in various food products as a functional ingredient. Biopolymer nanoparticles were assembled from sodium caseinate and gum arabic using electrostatic complexation in the presence of nonionic surfactant Tween 20. Fourier transform infrared spectroscopy was used to investigate the interactions of curcumin with protein, polysaccharide and surfactant. The effects of sodium caseinate (A), gum arabic (B), Tween 20 (C) and pH (D) on responses were studied using a three-level four-factor Box–Behnken design. For each response, a second-order polynomial model was developed. For optimum nanoencapsulation of curcumin, the variables concentration of sodium caseinate, concentration of gum arabic, pH and concentration of Tween 20 were 0.21, 0.5, 5 and 0.14 wt% respectively. The particles were characterized by ζ-potential measurement. Spectroscopic results and data modelling, showed interaction of curcumin with sodium caseinate, gum arabic and Tween 20. Also, the nonionic surfactant Tween 20, influenced the electrostatic interaction between sodium caseinate and gum arabic.
机译:这项研究的目标是将水溶性姜黄素包装不良,以便将其作为功能成分用于各种食品。在非离子型表面活性剂Tween 20存在下,使用酪蛋白酸钠和阿拉伯树胶组装生物聚合物纳米颗粒,采用非离子表面活性剂Tween 20。使用三级四因素Box-Behnken设计研究了酪蛋白酸钠(A),阿拉伯树胶(B),吐温20(C)和pH(D)对响应的影响。对于每个响应,开发了一个二阶多项式模型。为了获得最佳的姜黄素纳米胶囊包封率,酪蛋白酸钠的浓度,阿拉伯胶的浓度,pH和Tween 20的浓度分别为0.21、0.5、5和0.14 wt%。通过ζ电势测量来表征颗粒。光谱结果和数据建模表明姜黄素与酪蛋白酸钠,阿拉伯树胶和吐温20相互作用。而且,非离子表面活性剂吐温20影响酪蛋白酸钠和阿拉伯树胶之间的静电相互作用。

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