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首页> 美国卫生研究院文献>other >Plasmid Characterization and Chromosome Analysis of Two netF+ Clostridium perfringens Isolates Associated with Foal and Canine Necrotizing Enteritis
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Plasmid Characterization and Chromosome Analysis of Two netF+ Clostridium perfringens Isolates Associated with Foal and Canine Necrotizing Enteritis

机译：两种与驹和犬坏死性肠炎有关的netF +产气荚膜梭菌分离株的质粒表征和染色体分析

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The recent discovery of a novel beta-pore-forming toxin, NetF, which is strongly associated with canine and foal necrotizing enteritis should improve our understanding of the role of type A Clostridium perfringens associated disease in these animals. The current study presents the complete genome sequence of two netF-positive strains, JFP55 and JFP838, which were recovered from cases of foal necrotizing enteritis and canine hemorrhagic gastroenteritis, respectively. Genome sequencing was done using Single Molecule, Real-Time (SMRT) technology-PacBio and Illumina Hiseq2000. The JFP55 and JFP838 genomes include a single 3.34 Mb and 3.53 Mb chromosome, respectively, and both genomes include five circular plasmids. Plasmid annotation revealed that three plasmids were shared by the two newly sequenced genomes, including a NetF/NetE toxins-encoding tcp-conjugative plasmid, a CPE/CPB2 toxins-encoding tcp-conjugative plasmid and a putative bacteriocin-encoding plasmid. The putative beta-pore-forming toxin genes, netF, netE and netG, were located in unique pathogenicity loci on tcp-conjugative plasmids. The C. perfringens JFP55 chromosome carries 2,825 protein-coding genes whereas the chromosome of JFP838 contains 3,014 protein-encoding genes. Comparison of these two chromosomes with three available reference C. perfringens chromosome sequences identified 48 (~247 kb) and 81 (~430 kb) regions unique to JFP55 and JFP838, respectively. Some of these divergent genomic regions in both chromosomes are phage- and plasmid-related segments. Sixteen of these unique chromosomal regions (~69 kb) were shared between the two isolates. Five of these shared regions formed a mosaic of plasmid-integrated segments, suggesting that these elements were acquired early in a clonal lineage of netF-positive C. perfringens strains. These results provide significant insight into the basis of canine and foal necrotizing enteritis and are the first to demonstrate that netF resides on a large and unique plasmid-encoded locus.
机译：最近发现了一种新的形成β孔的毒素NetF，该毒素与犬和小马驹坏死性肠炎密切相关，应该会增进我们对A型产气荚膜梭菌相关疾病在这些动物中的作用的了解。目前的研究显示了两种netF阳性菌株JFP55和JFP838的完整基因组序列，分别从小马驹坏死性肠炎和犬出血性胃肠炎中回收。基因组测序使用单分子实时（SMRT）技术-PacBio和Illumina Hiseq2000完成。 JFP55和JFP838基因组分别包含一个3.34 Mb和3.53 Mb染色体，两个基因组都包含五个环状质粒。质粒注释显示三个新的基因组共有三个质粒，包括一个编码NetF / NetE毒素的tcp结合质粒，一个编码CPE / CPB2毒素的tcp结合质粒和一个假定的细菌素编码质粒。推定的形成β孔的毒素基因netF，netE和netG位于tcp偶联质粒的独特致病基因座中。产气荚膜梭菌的JFP55染色体携带2,825个蛋白质编码基因，而JFP838的染色体包含3,014个蛋白质编码基因。将这两个染色体与三个可用的参考产气荚膜梭菌染色体序列进行比较，分别确定了JFP55和JFP838独特的48个（〜247 kb）和81个（〜430 kb）区域。两个染色体中的这些不同基因组区域中的一些是噬菌体和质粒相关的区段。在两个分离株之间共有16个这些独特的染色体区域（〜69 kb）。这些共享区域中的五个形成了质粒整合片段的镶嵌体，表明这些元件是在netF阳性产气荚膜梭菌菌株的克隆谱系中早期获得的。这些结果提供了对犬和小马驹坏死性肠炎的基础的重要见解，并且是首次证明netF驻留在一个大而独特的质粒编码基因座上。

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