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Comparative analysis of carotenoid content in Momordica cochinchinensis (Cucurbitaceae) collected from Australia Thailand and Vietnam

机译:从澳大利亚泰国和越南采集的苦瓜中类胡萝卜素含量的比较分析

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摘要

Momordica cochinchinensis (Cucurbitaceae) is the richest source of lycopene and β-carotene of all known fruits but the influences of collection sites, variety and environment on carotenoid accumulation is unknown. This study analysed the carotenoid content of 44 M. cochinchinensis aril samples collected from Australia, Thailand and Vietnam using HPLC, UV–visible spectrophotometry and compared with the colorimetry method. The highest lycopene content was observed in samples collected from Ha Noi (7.76 mg/g) of Northern Vietnam and Lam Ha (6.45 mg/g) and Lam Dong (6.64 mg/g) provinces of Central Vietnam. The highest β-carotene content was observed in a sample from Nam Dinh (9.60 mg/g) in Northern Vietnam while a variety from Hoa Binh province in Northern Vietnam had high contents of both lycopene (5.17 mg/g) and β-carotene (5.66 mg/g). Lycopene content was higher in samples collected from low temperatures (<14 °C) and higher elevations whilst β-carotene content was greatest at temperatures between 27 and 33 °C. Crop improvement for increased lycopene and β-carotene requires rapid and accurate methods of quantification. All three analytical methods utilised were in agreement for lycopene quantification. The (a*/b*)2 transformed colour value resulted in more linear relationship for lycopene indicating that colorimetry method could potentially be developed to select lycopene rich fruits in the field.Electronic supplementary materialThe online version of this article (doi:10.1007/s13197-017-2719-0) contains supplementary material, which is available to authorized users.
机译:苦瓜是所有已知水果中番茄红素和β-胡萝卜素的最丰富来源,但收集地点,品种和环境对类胡萝卜素积累的影响尚不清楚。这项研究使用HPLC,紫外可见分光光度法分析了从澳大利亚,泰国和越南收集的44种天竺葵假种皮中类胡萝卜素的含量,并与比色法进行了比较。从越南北部的河内(7.76 mg / g)和越南中部的林河(6.45 mg / g)和林洞(6.64 mg / g)的省份收集的样品中观察到的番茄红素含量最高。在越南北部的南定(Nam Dinh)样品中观察到最高的β-胡萝卜素(9.60mg / g),而越南北部的Hoa Binh省的一个品种中番茄红素(5.17mg / g)和β-胡萝卜素的含量都很高( 5.66 mg / g)。从低温(<14°C)和更高的海拔收集的样品中番茄红素含量较高,而β-胡萝卜素含量在27至33°C的温度下最大。要提高番茄红素和β-胡萝卜素的产量,就需要快速而准确的定量方法。所使用的所有三种分析方法均符合番茄红素的定量要求。 (a * / b *) 2 变换后的色值导致番茄红素具有更多的线性关系,这表明可以使用比色法来选择该领域中富含番茄红素的水果。电子补充材料文章(doi:10.1007 / s13197-017-2719-0)包含补充材料,授权用户可以使用。

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