• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Frontiers in Plant Science >Polyamine and Its Metabolite H2O2 Play a Key Role in the Conversion of Embryogenic Callus into Somatic Embryos in Upland Cotton (Gossypium hirsutum L.)
【2h】

Polyamine and Its Metabolite H2O2 Play a Key Role in the Conversion of Embryogenic Callus into Somatic Embryos in Upland Cotton (Gossypium hirsutum L.)

机译：多胺及其代谢产物H2O2在陆地棉（Gossypium hirsutum L.）的胚性愈伤组织转化为体细胞胚中起关键作用。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The objective of this study was to increase understanding about the mechanism by which polyamines (PAs) promote the conversion of embryogenic calli (EC) into somatic embryos in cotton (Gossypium hirsutum L.). We measured the levels of endogenous PAs and H2O2, quantified the expression levels of genes involved in the PAs pathway at various stages of cotton somatic embryogenesis (SE), and investigated the effects of exogenous PAs and H2O2 on differentiation and development of EC. Putrescine (Put), spermidine (Spd), and spermine (Spm) significantly increased from the EC stage to the early phase of embryo differentiation. The levels of Put then decreased until the somatic embryo stage whereas Spd and Spm remained nearly the same. The expression profiles of GhADC genes were consistent with changes in Put during cotton SE. The H2O2 concentrations began to increase significantly at the EC stage, during which time both GhPAO1 and GhPAO4 expressions were highest and PAO activity was significantly increased. Exogenous Put, Spd, Spm, and H2O2 not only enhanced embryogenic callus growth and embryo formation, but also alleviated the effects of D-arginine and 1, 8-diamino-octane, which are inhibitors of PA synthesis and PAO activity. Overall, the results suggest that both PAs and their metabolic product H2O2 are essential for the conversion of EC into somatic embryos in cotton.
机译：这项研究的目的是增进对多胺（PAs）促进棉花（Gossypium hirsutum L.）胚发生愈伤组织（EC）转化为体细胞胚的机制的了解。我们测量了内源性PA和H2O2的水平，定量了在棉花体细胞胚发生（SE）各个阶段参与PAs途径的基因的表达水平，并研究了外源PA和H2O2对EC分化和发育的影响。从EC阶段到胚胎分化的早期，腐胺（Put），亚精胺（Spd）和亚精胺（Spm）显着增加。然后，Put的水平下降直至体细胞胚胎阶段，而Spd和Spm保持几乎相同。 GhADC基因的表达谱与棉花SE期间Put的变化一致。在EC阶段，H2O2浓度开始显着增加，在此期间，GhPAO1和GhPAO4的表达均最高，而PAO活性则显着增加。外源性Put，Spd，Spm和H2O2不仅增强了胚性愈伤组织的生长和胚的形成，而且减轻了D-精氨酸和1，8-二氨基辛烷的作用，它们是PA合成和PAO活性的抑制剂。总体而言，这些结果表明，PA及其代谢产物H2O2对EC转化为棉花中的体细胞胚至关重要。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号