首页> 美国卫生研究院文献>The Journal of Experimental Medicine >GROWTH CHARACTERISTICS OF MONKEY KIDNEY CELL STRAINS LLC-MK1 LLC-MK2 AND LLC-MK2(NCTC-3196) AND THEIR UTILITY IN VIRUS RESEARCH
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GROWTH CHARACTERISTICS OF MONKEY KIDNEY CELL STRAINS LLC-MK1 LLC-MK2 AND LLC-MK2(NCTC-3196) AND THEIR UTILITY IN VIRUS RESEARCH

机译:猴子肾细胞株LLC-MK1LLC-MK2和LLC-MK2(NCTC-3196)的生长特性及其在病毒研究中的实用性

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摘要

The establishment of two strains of rhesus monkey kidney cells in continuous tissue culture, the development of a subline adapted to chemically defined medium, and the isolation of several clonal derivatives were described. Growth characteristics, chromosome numbers, malignant potentiality, and freeze storage data are presented. The cells were studied for their sensitivity to a large number of viruses and were extensively compared with primary cultures of monkey kidney cells for sensitivity to poliovirus. The cell strains were not sensitive to all the viruses which could be grown in primary cultures of the same tissue but were susceptible to most of them. In some instances an advantage to the use of the cell strain for certain viruses was noted.
机译:描述了在连续组织培养中建立的两种恒河猴肾细胞株,开发了适合化学成分确定的亚系,并分离了几种克隆衍生物。给出了生长特征,染色体数目,恶性潜能和冻存数据。研究了这些细胞对多种病毒的敏感性,并与猴肾细胞的原代培养物对脊髓灰质炎病毒的敏感性进行了广泛比较。细胞株对所有可能在同一组织的原代培养物中生长的病毒都不敏感,但对大多数病毒敏感。在某些情况下,注意到了将细胞株用于某些病毒的优势。

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