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首页> 美国卫生研究院文献>Frontiers in Plant Science >Development of genome-wide informative simple sequence repeat markers for large-scale genotyping applications in chickpea and development of web resource
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Development of genome-wide informative simple sequence repeat markers for large-scale genotyping applications in chickpea and development of web resource

机译：鹰嘴豆大规模基因分型应用的全基因组信息简单序列重复标记的开发和网络资源的开发

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Development of informative polymorphic simple sequence repeat (SSR) markers at a genome-wide scale is essential for efficient large-scale genotyping applications. We identified genome-wide 1835 SSRs showing polymorphism between desi and kabuli chickpea. A total of 1470 polymorphic SSR markers from diverse coding and non-coding regions of the chickpea genome were developed. These physically mapped SSR markers exhibited robust amplification efficiency (73.9%) and high intra- and inter-specific polymorphic potential (63.5%), thereby suggesting their immense use in various genomics-assisted breeding applications. The SSR markers particularly derived from intergenic and intronic sequences revealed high polymorphic potential. Using the mapped SSR markers, a wider functional molecular diversity (16–94%, mean: 68%), and parentage- and cultivar-specific admixed domestication pattern and phylogenetic relationships in a structured population of desi and kabuli chickpea genotypes was evident. The intra-specific polymorphism (47.6%) and functional molecular diversity (65%) potential of polymorphic SSR markers developed in our study is much higher than that of previous documentations. Finally, we have developed a user-friendly web resource, Chickpea Microsatellite Database (CMsDB; ), which provides public access to the data and results reported in this study. The developed informative SSR markers can serve as a resource for various genotyping applications, including genetic enhancement studies in chickpea.
机译：在全基因组范围内开发信息多态性简单序列重复（SSR）标记对于有效的大规模基因分型应用至关重要。我们鉴定出全基因组1835 SSR，显示在desi和kabuli鹰嘴豆之间存在多态性。鹰嘴豆基因组的不同编码和非编码区域共开发了1470个多态性SSR标记。这些物理定位的SSR标记表现出强大的扩增效率（73.9％）和高的种内和种间多态性潜力（63.5％），从而表明它们在各种基因组辅助育种应用中的大量使用。特别地从基因间和内含子序列衍生的SSR标记显示出高多态性潜力。使用映射的SSR标记，可以在结构化的desi和kabuli鹰嘴豆基因型人群中发现更广泛的功能分子多样性（16–94％，平均值：68％），以及亲本和品种特异性的混合驯化模式和系统发生关系。在我们的研究中开发的多态SSR标记的种内多态性（47.6％）和功能分子多样性（65％）的潜力远高于以前的文献。最后，我们开发了一个用户友好的Web资源，鹰嘴豆微卫星数据库（CMsDB;），该数据库提供对本研究报告的数据和结果的公共访问。已开发的信息丰富的SSR标记可作为各种基因分型应用的资源，包括鹰嘴豆的遗传增强研究。

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