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Genetic Diversity Strategy for the Management and Use of Rubber Genetic Resources: More than 1000 Wild and Cultivated Accessions in a 100-Genotype Core Collection

机译:橡胶遗传资源管理和利用的遗传多样性策略:100个基因型核心收藏物中的1000多个野生和栽培种质

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摘要

The rubber tree [Hevea brasiliensis (Willd. ex Adr. de Juss.) Muell. Arg.] is the only plant species worldwide that is cultivated for the commercial production of natural rubber. This study describes the genetic diversity of the Hevea spp. complex that is available in the main ex situ collections of South America, including Amazonian populations that have never been previously described. Genetic data were analyzed to determine the genetic structure of the wild populations, quantify the allelic diversity and suggest the composition of a core collection to capture the maximum genetic diversity within a minimal sample size. A total of 1,117 accessions were genotyped with 13 microsatellite markers. We identified a total of 408 alleles, 319 of which were shared between groups and 89 that were private in different groups of accessions. In a population structure and principal component analysis, the level of clustering reflected a primary division into the following two subgroups: cluster 1, which consisted of varieties from the advanced breeding germplasm that originated from the Wickham and Mato Grosso accessions; and cluster 2, which consisted of the wild germplasm from the Acre, Amazonas, Pará and Rondônia populations and Hevea spp. The analyses revealed a high frequency of gene flow between the groups, with the genetic differentiation coefficient (GST) estimated to be 0.018. Additionally, no distinct separation among the H. brasiliensis accessions and the other species from Amazonas was observed. A core collection of 99 accessions was identified that captured the maximum genetic diversity. Rubber tree breeders can effectively utilize this core collection for cultivar improvement. Furthermore, such a core collection could provide resources for forming an association panel to evaluate traits with agronomic and commercial importance. Our study generated a molecular database that should facilitate the management of the Hevea germplasm and its use for subsequent genetic and genomic breeding.
机译:橡胶树[巴西橡胶树(Weved。ex Adr。de Juss。)Muell。 Arg。]是全世界唯一用于商业生产天然橡胶的植物。这项研究描述了橡胶树种的遗传多样性。该复合体可在南美主要的非原生境收藏中使用,包括以前从未描述过的亚马逊种群。分析了遗传数据,以确定野生种群的遗传结构,量化了等位基因多样性,并提出了核心集合的组成,以在最小样本量内捕获最大的遗传多样性。用13个微卫星标记对1,117份种质进行基因分型。我们共鉴定了408个等位基因,其中319个在组之间共享,而89个在不同的登录组中是私有的。在种群结构和主成分分析中,聚类的水平反映出主要分为以下两个亚组:聚类1,由来自Wickham和Mato Grosso品系的先进育种种的品种组成;聚类2,由英亩,亚马逊,帕拉和朗多尼亚种群和三叶橡胶属的野生种质组成。分析显示两组之间的基因流动频率很高,遗传分化系数(GST)估计为0.018。此外,没有观察到巴西利亚嗜血杆菌的种和其他来自亚马逊亚种的分离。确定了收集了最大遗传多样性的99个种质的核心收藏。橡胶树育种者可以有效地利用这一核心资源进行品种改良。此外,这样的核心收藏可以提供资源来组成一个协会小组,以评估具有农艺和商业重要性的性状。我们的研究产生了一个分子数据库,该数据库应该有助于橡胶树种质的管理及其在随后的遗传和基因组育种中的用途。

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